In-vitro and In-vivo Identification, Absorbtion and Metabolism Network Analysis of Filifolium sibiricum Flavonoids Dropping Pill by UHPLC-Q-TOF-MS-Reference-Cited by-同舟云学术

In-vitro and In-vivo Identification, Absorbtion and Metabolism Network Analysis of Filifolium sibiricum Flavonoids Dropping Pill by UHPLC-Q-TOF-MS

Published:2022-12 Issue:14 Volume:23 Page:1143-1155
ISSN:1389-2002
Container-title:Current Drug Metabolism
language:en
Short-container-title:CDM

Author:

Ma Rui-Ting¹²,Han Ji-Xin³,Qiao Jun-Chan⁴,Tong Li-Jun¹,Chen Li-Xia¹

Affiliation:

1. The Inner Mongolia Mental Health Center, Hohhot 010010, China

2. Department of Life Science, Nanjing Normal University, Nanjing, 210024, China

3. Department of Chemical Engineering, Inner Mongolia Vocational College of Chemical Technology, Hohhot, 010050, China

4. Pharmacy Department, Inner Mongolia Medical University, Hohhot 010110, China

Abstract

Background: Filifolium sibiricum flavonoids dropping pill (FSFp), a unique Chinese Filifolii sibirici herba extract preparation, has the potential as an alternative therapy against S. aureus infection (SA) and antiinfection. However, its chemical composition and in vivo metabolism characteristics remain unknown, which limits its clinical application. Methods: Here, we aimed to understand the in vitro and in vivo material basis of FSFp. Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) was used to identify chemicals in FSFp as well as its phase I and phase II reaction metabolites in plasma, urine and feces. Results: A total of 38 chemicals were characterized in FSFp, including 22 flavonoids, 10 organic acids, 3 chromones, 1 aromatic ketone, 1 coumarin, and 1 ligan. After analysis of the drugged bio-samples, a total of 21 compounds were found in urine, and 16 of them were found in feces, but only one was found in plasma. In addition, 56 FSFp-related metabolites were characterized, of which 56 were in urine, 4 in feces, and 8 in plasma. Conclusion: This is the first comprehensive research of FSFp on chemical constituents and metabolic profiles. It was expected that this study would offer reliable support for further investigation of FSFp.

Funder

Inner Mongolia Science and Technology Project

Inner Mongolia Health Science and Technology Plan Project

Publisher

Bentham Science Publishers Ltd.

Subject

Clinical Biochemistry,Pharmacology

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