Affiliation:
1. Department of Pharmacology (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular
Medicine Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, China
2. Science
and Technology Park, Harbin Medical University, Harbin, China
3. Research Unit of Noninfectious Chronic Diseases in Frigid
Zone, Chinese Academy of Medical Sciences, Harbin, China
Abstract
Background:
Colorectal cancer (CRC) is the most common type of gastrointestinal tumor, but the
available pharmacological treatment is insufficient. As a traditional Chinese medicine, the green walnut husks
(QLY) exhibit anti-inflammatory, analgesic, anti-bacterial and anti-tumor effects. However, the effects and
molecular mechanisms of QLY extracts on CRC were not yet made known.
Objective:
This study aims to provide efficient and low toxicity drugs for the treatment of CRC. The purpose of
this study is to explore the anti-CRC effect and mechanism of QLY, providing preliminary data support for clinical
research of QLY.
Methods:
Western blotting, Flow cytometry, immunofluorescence, Transwell, MTT, Cell proliferation assay,
and xenograft model were used to perform the research.
Results:
In this study, the potential of QLY to inhibit the proliferation, migration invasion and induce apoptosis
of the mouse colorectal cancer cell line CT26 in vitro was identified. The xenograft tumor model of CRC noted
that QLY suppressed tumor growth without sacrificing body weight in mice. In addition, QLY-induced apoptosis
in tumor cells through NLRC3/PI3K/AKT signaling pathway was revealed.
Conclusions:
QLY regulates the levels of mTOR, Bcl-2 and Bax by affecting the NLRC3/PI3K/AKT pathway
to promote apoptosis of tumor cells, suppressing cell proliferation, invasion and migration, and subsequently
preventing the progression of colon cancer.
Funder
Postdoctoral Research Initiation Fund
CAMS Innovation Fund for Medical Sciences
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Pharmacology
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