Affiliation:
1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract
Introduction:
While PCR has been recognized as one of the appropriate ways to diagnose infectious
diseases, Loop-mediated isothermal amplification (LAMP) which is a nucleic acid amplification method, can
be considered as an alternative to PCR, and it is faster, cost-effective, and easier to perform than nested PCR.
Patients and Methods:
Keywords were searched in PubMed/MEDLINE, Scopus and Institute for Scientific Information
Web of Science, as well as the search engine of Google Scholar. Keywords included PCR, LAMP,
RAA, RPA, Virus and COVID-19.
Results:
LAMP technology has been extensively applied for the detection of human pathogenic bacteria, crop
pests, pathogenic organisms and components in meat products. A new isotheral method, Recombinase polymerase
amplification (RPA), can amplify the DNA as well as RPA. RPA involves benefits of isothermal PCR
as well as simplicity and rapid amplification. Recombinase aided amplification (RAA) assay has been favorably
used in the detection of bacterial and viral pathogens and solved the technical difficulties posed by DNA amplification
methods because it does not need thermal denaturation of the template and involves a low and constant
temperature.
Conclusions:
Reverse transcription polymerase chain reaction, digital PCR, LAMP, nicking endonuclease amplification
reaction, recombinase polymerase amplification, and clustered regularly interspaced short palindromic
repeats are different nucleic acid amplification tests of COVID-19. LAMP methods can be more specific than
qPCR and immunoassays. The LAMP assay can be applied for rapid detection of SARS-CoV, MERS-CoV,
SARS-CoV-2, and influenza, because LAMP is a highly sensitive and specific DNA/RNA amplification technique.
Funder
Natural Science Foundation of Beijing, China
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Pharmacology
Cited by
12 articles.
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