Antiulcer and Antioxidant Activity of a Lectin from Mucuna pruriens Seeds on Ethanol- induced Gastropathy: Involvement of Alpha-2 Adrenoceptors and Prostaglandins

Author:

Pinto Isabela Ribeiro1,Chaves Hellíada V.1,Vasconcelos Auriana S.2,de Sousa Francisca Clea F2,Santi-Gadelha Tatiane3,de Lacerda José Thalles Jocelino Gomes3,Ribeiro Kátia Alves1,Freitas Raul Sousa1,Maciel Lara Mendes4,Filho Samuel Mateus Pereira4,Viana Ana Flávia Seraine Custódio2,de Almeida Gadelha Carlos Alberto3,Filho Gerardo Cristino1,de Paulo Teixeira Pinto Vicente1,Pereira Karuza Maria Alves5,Rodrigues e Silva Antonio Alfredo1,Bezerra Mirna Marques1

Affiliation:

1. Federal University of Ceara, Master of Biotechnology Degree Program, Sobral - Ceara, Brazil

2. Federal University of Ceara, Department of Pharmacology, Fortaleza - Ceara, Brazil

3. Federal University of Paraiba, Department of Molecular Biology, Joao Pessoa - Paraiba, Brazil

4. Federal University of Ceara, Faculty of Medicine, Sobral - Ceara, Brazil

5. Federal University of Ceara, Department of Morphology, Fortaleza - Ceara, Brazil

Abstract

Ethnopharmacological Relevance:Mucuna pruriens (Mp) belongs to Leguminosae family, it is native of tropical regions and used to treat several maladies such as urinary, neurological, and menstruation disorders, constipation, edema, fever, tuberculosis, ulcers, diabetes, arthritis, dysentery, and cardiovascular diseases. Mp seeds are rich in bioactive compounds, for instance, lectins, a heterogeneous group of proteins and glycoproteins with a potential role as therapeutic tools for several conditions, including gastric disorders. This study investigated the acute toxicity, gastroprotective, and antioxidant activities of a lectin from Mucuna pruriens seeds (MpLec) on ethanol-induced gastropathy model in mice.Materials & Methods:Mice received MpLec (5 or 10 mg/kg; i.v.) and were observed for acute toxicity signs; in another experimental series, mice were pre-treated with MpLec (0.001; 0.01 or 0.1 mg/kg, i.v.), ranitidine (80 mg/kg, p.o.), or saline (0.3 mL/30g, i.v.) before ethanol 99.9% (0.2 mL/animal, p.o.), and euthanized 30 min after ethanol challenge. Macroscopic and microscopic gastric aspects, biochemical parameters (tissue hemoglobin levels, iron-induced lipid peroxidation, GSH content, SOD activity, and gastric mucosal PGE2) were measured. Additionally, pharmacological tools (yohimbine, indomethacin, naloxone, L-NAME) were opportunely used to clarify MpLec gastroprotective mechanisms of action.Results:No toxicity signs nor death were observed at acute toxicity tests. MpLec reduced ethanol-induced gastric damage, edema, and hemorrhagic patches formation, as well as decreased lipid peroxidation, SOD activity, and increased GSH content. Yohimbine and indomethacin prevented MpLec effects, suggesting the involvement of alpha-2 adrenoceptors and prostaglandins in the MpLec-mediated effects.Conclusion:MpLec does not present toxicity signs and shows gastroprotective and antioxidant activities via alpha-2 adrenoceptors and prostaglandins in the ethanol-induced gastropathy model.

Publisher

Bentham Science Publishers Ltd.

Subject

Drug Discovery,Pharmacology

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