The Renoprotective and Anti-Inflammatory Effects of Human Urine-Derived Stem Cells on Acute Kidney Injury Animals

Abstract

Background: Acute Kidney Injury (AKI) is defined as a sudden loss of kidney function, which is often caused by drugs, toxins, and infections. The large spectrum of AKI implies diverse pathophysiological mechanisms. In many cases, AKI can be lethal, and kidney replacement therapy is frequently needed. However, current treatments are not satisfying. Developing novel therapies for AKI is essential. Adult stem cells possess regenerative ability and play an important role in medical research and disease treatment. background: Acute kidney injury (AKI) is defined by a sudden loss of kidney function, which is often caused by drugs, toxins, and infections. The large spectrum of AKI implies diverse pathophysiological mechanisms. In many cases, AKI can be lethal, and kidney replacement therapy is frequently needed. However, current treatments are not satisfying. Methods: In this study, we isolated and characterized a distinct human urine-derived stem cell, which expressed both proximal tubular cell and mesenchymal stem cell genes as well as certain unique genes. objective: Developing novel therapies for AKI is essential. Adult stem cells possess regenerative powers and play an important role in medical research and disease treatment. Results: It was found that these cells exhibited robust protective effects on tubular cells and anti- inflammatory effects on macrophages in vitro. In an ischemia-reperfusion-induced acute kidney injury NOD-SCID mouse model, transplantation of USCs significantly protected the kidney morphology and functions in vivo. method: Here we isolated and characterized a distinct human urine-derived stem cell, which expresses both proximal tubular cell and mesenchymal stem cell genes as well as certain unique genes. Conclusion: In summary, our results highlighted the effectiveness of USCs in protecting from PTC injury and impeding macrophage polarization, as well as the secretion of pro-inflammatory interleukins, suggesting the potential of USCs as a novel cell therapy in AKI. result: We discovered that these cells exhibited robust protective effects on tubular cells and anti-inflammatory effects on macrophages in vitro. In an ischemia-reperfusion induced acute kidney injury mouse model, transplantation of USCs significantly protected the kidney morphology and functions in vivo.