Preparation of Decellularized Lung Matrix Scaffolds and Evaluation of its Effect on Endometrial Stem Cell Differentiation into Lung Epithelial-like Cells-Reference-Cited by-同舟云学术

Preparation of Decellularized Lung Matrix Scaffolds and Evaluation of its Effect on Endometrial Stem Cell Differentiation into Lung Epithelial-like Cells

Published:2024-06-04 Issue: Volume:19 Page:
ISSN:1574-888X
Container-title:Current Stem Cell Research & Therapy
language:en
Short-container-title:CSCR

Author:

Mohamadnia Abdolreza¹,Bagherzadi Kambiz²,Hasanzadeh Elham³,Noori Afshin⁴,Jamaati Hamidreza¹,Dizaji Mehdi Kazempour⁵,Ai Arman⁶,Jahromi Hossein Kargar⁷,Afsartala Zohreh⁸,Ai Jafar⁹,bordbar Sima¹⁰¹¹,Bahrami Naghmeh⁹¹²

Affiliation:

1. Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

2. Anesthesiology Specialty and Pain Fellowship. Islamic Azad University, Tehran, Iran

3. Immunogenetics Research Center, Department of Tissue Engineering & Regenerative Medicine, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran

4. Cardio-Pulmonary Institute, Department of Pulmonary and Critical Care Medicine and Infectious Diseases, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research, Justus-Liebig University Giessen, 35392, Giessen, Germany

5. Biostatistics Department, Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

7. Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran

8. Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Science, Tehran, Iran

9. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

10. Anatomical Sciences & Cognitive Neuroscience Department, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

11. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

12. Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Background: The use of decellularized tissues as cell culture scaffolds is an exciting new direction in regenerative medicine because they may provide the instructional context for cell development and function. Objective: Building a scaffold with biomimetic chemical, structural, and functional features is crucial for lung tissue healing. Due to the diverse nature of their structure, a decellularized lung matrix derived from both allogeneic and xenogeneic sources is regarded as an ideal scaffold for lung regeneration. Methods: By decellularizing rat lungs using a combination of chemical and physical methods, we were able to build a scaffold for lung tissue engineering. A decellularized lung was tested for DNA content, and histologically, it was shown to be totally free of cells after using this process. Results: The decellularized lung was biocompatible for the growth of human endometrial stem cells (hEnSCs), as evidenced by scanning electron microscopy (SEM), an MTS assay, and Hematoxylin and Eosin (H&E) staining. In addition, we found that decellularized scaffolds induced lung epithelial cell differentiation from EnSCs by upregulating a subset of genes. Lung epithelial cell development from stem cells was also induced by decellularized scaffolds, as shown by an increase in the expression of a gene that is only expressed in lung epithelial cells. The strong level of acellularized scaffold affinity for cell adhesion, proliferation, and growth was also shown to promote lung lesion regeneration in rats after four weeks of treatment, according to in vivo research. Conclusion: In summary, the decellularized lung scaffold that has been developed offers a highly accurate framework for the effective restoration of lung tissues. These scaffolds prove to be valuable tools for investigating the mechanisms by which the tissue microenvironment facilitates the growth, differentiation, and function of lung epithelial-like cells, ultimately contributing to the beneficial outcomes of lung repair.

Publisher

Bentham Science Publishers Ltd.