Macroporous Nanostructured Calcium Phosphate/Chitosan-Gelatin Composite Bone Tissue Engineering Scaffold

Abstract

Background: Tissue engineering is an emerging technology developed for the therapeutic reconstruction of damaged tissue. Objective: In this study, a ceramic/polymer nanocomposite bone tissue engineering scaffold was prepared by coating a tetracalcium phosphate/dicalcium phosphate mixture slurry on a porous 3D chitosan-gelatin construction. Methods: The phase composition, structural groups, and morphological aspects of the samples were characterized. Furthermore, the 3D composite scaffold was immersed in simulated body fluid (SBF) solution at 37ºC for various periods to track its compositional and structural changes. Results: Based on the results, the coated layer is composed of needle-like carbonated apatite nanosized crystals with some tetracalcium phosphate/dicalcium phosphate initial materials. The nanocomposite was porous with an average macropore size of about 410 μm. The in vitro tests revealed that the composition of the coated layer tends to be apatite crystals, which are similar to natural bone in terms of chemistry and morphology. Conclusion: The results suggest that a simple coating of chitosan-gelatin scaffolds using reactive calcium phosphate particles may introduce a novel nanocomposite scaffold with improved mechanical strength, bioactivity, and osteoconductivity.