Affiliation:
1. Department of Medical Oncology, The First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, China
Abstract
Background:
Long-chain acyl-coenzyme A synthases (ACSLs) are responsible
for the catalysis of fatty acids into their corresponding fatty acyl-CoAs. The dysregulation
of ACSLs has been increasingly recognized in cancer patients. However, the function
of ACSL6 in triple-negative breast cancer (TNBC) is still completely unknown.
Methods:
In this study, immunohistochemistry was applied to detect ACSL6 protein expression
using a TNBC tissue microarray. Additionally, the mRNA levels of ACSL6 in
human normal tissues and pancancer tissues were analyzed using Genotype Tissue Expression
(GTEx) datasets and The Cancer Genome Atlas (TCGA) database. The correlations
between the levels of ACSL6 expression and clinical characteristics were analyzed.
The survival analysis of ACSL6 in TNBC was carried out using the Kaplan‒Meier
Plotter online tool. Associations of ACSL6 with immune infiltration analyses were
conducted using the ESTIMATE, CIBERSORT, and TISIDB databases. The relationship
between ACSL6 and sensitivity to drugs was analyzed from Genomics of Drug Sensitivity
in Cancer (GDSC).
Results:
The results indicated a significant increase in ACSL6 expression in TNBC tissues
compared to adjacent normal tissues. However, high ACSL6 expression was significantly
associated with favorable survival outcomes in TNBC patients. Enrichment analysis
revealed that coexpressed genes of ACSL6 were significantly enriched in various immunity
processes. ACSL6 was positively correlated with the infiltration of memory
CD4 T cells, while a negative correlation was found between ACSL6 and M2
macrophages and resting dendritic cells. Further analysis revealed that high levels of ACSL6
correlated with increased survival outcomes in cancer patients who received immunotherapy.
Conclusion:
Altogether, the current findings highlight the potential value of ACSL6 as
a diagnostic and prognostic marker in the treatment of TNBC.
Publisher
Bentham Science Publishers Ltd.
Cited by
1 articles.
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