Affiliation:
1. Department of Pediatrics, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010000, China
Abstract
Objective:
In this study, we employed an in vitro culturing technique to investigate the
impact of p53 on the modulation of growth-associated protein-43 (GAP-43) within the primary
cortical neurons of rat specimens.
Methods:
(1) Within the first 24 hours after birth, the bilateral cortex was extracted from newborn
Wistar rats and primary cortical neurons were cultured and identified. (2) The changes in the mRNA
and protein expressions of GAP-43 induced by p53 in rat primary cortical neurons cultured in
vitro were identified utilizing real-time polymerase chain reaction and western blot techniques.
Results:
(1) Lentiviral transfection of p53 within primary cortical neurons of rats elicited elevated
levels of both mRNA and protein expressions of GAP-43, consequently culminating in a noteworthy
augmentation of p53 expression. (2) The introduction of a p53 inhibitor in rat primary cortical
neurons resulted in a reduction in both mRNA and protein expressions of GAP-43.
Conclusion:
Within primary rat cortical neurons, p53 has the potential to prompt an augmentation
in both the transcriptional and protein expression levels of the GAP-43 protein.
Funder
Natural Science Foundation of Inner Mongolia Autonomous Region
Major Project of Inner Mongolia Medical University
Youth Science and Technology Talents Program-Class A
Major Scientific Research Project of Affiliated Hospital of Inner Mongolia Medical University
Inner Mongolia Autonomous Region Neurological Disease Clinical Medicine Research Center
Inner Mongolia Science and Technology Department
National Natural Science Foundation of China
Publisher
Bentham Science Publishers Ltd.