Differential miRNA Expression Profiles in Cumulus and Mural Granulosa Cells from Human Pre-ovulatory Follicles
Author:
Andrei Daniela1, Nagy Roland A.1, van Montfoort Aafke1, Tietge Uwe2, Terpstra Martijn3, Kok Klaas3, van den Berg Anke4, Hoek Annemieke1, Kluiver Joost4, Donker Rogier1
Affiliation:
1. Department of Obstetrics and Gynecology, University of Groningen, University Medical Centre Groningen, Groningen, Netherlands 2. Department of Pediatrics, University of Groningen, University Medical Centre Groningen, Groningen, Netherlands 3. Department of Genetics, University of Groningen, University Medical Centre Groningen, Groningen, Netherlands 4. Department of Pathology and Medical Biology, University of Groningen, University Medical Centre Groningen, Groningen, Netherlands
Abstract
Background:
Mural Granulosa Cells (MGCs) and Cumulus Cells (CCs) are two specialized
cell types that differentiate from a common progenitor during folliculogenesis. Although these two
cell types have specialized functions and gene expression profiles, little is known about their microRNA
(miRNA) expression patterns.
Objective:
To describe the miRNA profile of mural and cumulus granulosa cells from human preovulatory
follicles.
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Methods: Using small RNA sequencing, we defined the miRNA expression profiles of human primary
MGCs and CCs, isolated from healthy women undergoing ovum pick-up for in vitro Fertilization
(IVF).
Results:
Small RNA sequencing revealed the expression of several hundreds of miRNAs in MGCs
and CCs with 53 miRNAs being significantly differentially expressed between MGCs and CCs. We
validated the differential expression of miR-146a-5p, miR-149-5p, miR-509-3p and miR-182-5p by
RT-qPCR. Analysis of proven targets revealed 37 targets for miR-146a-5p, 43 for miR-182-5p, 2 for
miR-509-3p and 9 for miR-149-5p. Gene Ontology (GO) analysis for these 4 target gene sets revealed
enrichment of 12 GO terms for miR-146a-5p and 10 for miR-182-5p. The GO term ubiquitin-like protein
conjugation was enriched within both miRNA target gene sets.
We generated miRNA expression profiles for MGCs and CCs and identified several differentially
expressed miRNAs.
Publisher
Bentham Science Publishers Ltd.
Subject
Orthopedics and Sports Medicine,Emergency Medicine,General Medicine
Reference17 articles.
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