Affiliation:
1. Department of Biology, Faculty of Science, University of Sistan and Baluchestan, Zahedan, Iran
Abstract
Background:
Protein misfolding can lead to aggregation and these protein aggregates are
a fundamental cause of many neurodegenerative disorders such as Alzheimer's, Parkinson's,
Huntington's, Prion disease and amyotrophic lateral sclerosis. In recent years, a wide variety of
natural compounds have been investigated as protein aggregation inhibitors. Many investigations
have reported the therapeutic effects of botanicals constituents and their derivatives in neurodegenerative
diseases.
Objective:
In this study, we examined the effect of Perovskia abrotanoides Karel (P. abrotanoides)
root extract on the 1,4-dithiothreitol (DTT)-induced aggregation of proteins.
Methods:
The anti-aggregation ability of P. abrotanoides root extract was studied using visible absorption
spectroscopy (light scattering), fluorescence spectroscopy, and circular dichroism (CD)
spectroscopy.
Results:
The protective effect of P. abrotanoides root extract was varied in the three different-sized
proteins (insulin, α-lactalbumin, and ovotransferrin).
Conclusion:
The results showed that P. abrotanoides root extract was able to inhibit protein aggregations
in a concentration-dependent manner due to the interaction of P. abrotanoides root extract
with hydrophobic area of proteins.
Publisher
Bentham Science Publishers Ltd.
Subject
Complementary and alternative medicine,Drug Discovery
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