Affiliation:
1. Department of Botany, Prof. Ramkrishna More College, Savitribai Phule Pune University, Akurdi, Pune 411044, India
2. Department of Biotechnology, Modern College of Arts, Science and Commerce, Savitribai Phule Pune University, Ganeshkhind, Pune 411016, India
Abstract
Background:
Eulophia nuda, is a highly medicinal orchid with strong antioxidant and
anticancer potentials in traditional systems of medicine. But few reports are available on the scientific
validation. The aim of the study was to investigate phytochemical constituents, antioxidant and
cytotoxic efficacies of extracts of Eulophia nuda, and the underlying mechanisms-of-action via upregulation
of nuclear transcription factor-erythroid-2 related factor (Nrf2) and hemeoxygenase-1
(HO-1) pathways.
Methods:
Petroleum Ether (PEE), Ethyl Acetate (EAE), Methanol (ME) and Aqueous Methanol
(AqME) extracts of shade dried tubers were obtained and concentrated in vacuo. Total phenols, flavonoids,
condensed tannins, ascorbic acid and carotenoids were estimated from the extracts using
standard methods. Antioxidant activities of extracts were determined by total antioxidant, FRAP,
ABTS, DPPH, OH, H2O2, NO, O2
·- radical scavenging assays. Cytotoxicity of EAE and ME were
assessed against MCF7 cells in vitro. LC-ESI/MS profiling of EAE was carried out. Quantitative
Real-Time (qRT) PCR was used for the expression analysis of Nrf2 and HO1 genes in EAE-treated
MCF7 cells.
Results:
In vitro models confirmed strong dose-dependent antioxidant and free-radical scavenging
potencies of E. nuda tuber extracts. Overall antioxidant efficacies were in the order EAE > ME >
AqME > PEE. EAE showed striking cytotoxicity followed by ME (0.86% and 5.17% cell survival
at 1000 µg ml-1, respectively). LC-ESI/MS profiling of most potent extract EAE revealed 37 identified
compounds including catechin, taxifolin, tocopherol, trigallic acid and chlorogenic acid, all
known for their strong antioxidant/anticancer properties. Expression levels of Nrf2 and HO1 genes
were up-regulated in MCF7 cells beyond 50 μg ml-1 extract concentration with > 2-fold increase at
200 µg ml-1 EAE.
Conclusion:
The data demonstrated that E. nuda extracts possess strong free radical scavenging and
antioxidant efficacies and the mechanism of action may be via inducing Nrf2 and HO-1.
Publisher
Bentham Science Publishers Ltd.
Subject
Complementary and alternative medicine,Drug Discovery
Reference63 articles.
1. Nanekar V.; Shriram V.; Kumar V.; Kishor P.B.K.; Asymbiotic in vitro seed germination and seedling development of Eulophia nuda Lindl. An endangered medicinal orchid. Proc Natl Acad Sci, India, Sect B Biol Sci 2014,84,837-846
2. Shriram V.; Nanekar V.; Kumar V.; Kishor P.B.K.;
In vitro regeneration and ploidy level analysis of Eulophia ochreata Lindl. Indian J Exp Biol 2014,52,1112-1121
3. Kumar V.; Lemos M.; Sharma M.; Shriram V.; Antioxidant and DNA damage protecting activities of Eulophia nuda Lindl. Free Radic Antioxid 2013,3,55-60
4. Singh A.; Duggal, S. Medicinal orchids - An overview. Ethnobot Leafl 2009,13,399-412
5. Jagdale S.P.; Shimpi S.; Chachad D.; Pharmacological studies of Salep. J Herb Med Toxicol 2009,3,153-156
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