Affiliation:
1. University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, 160014, India
Abstract
Background:
Ferula sumbul Hook. (Umbelliferae) roots have been traditionally used as
sedative in nervous disorders.
Objective:
The present study identifies the components of essential oil of F. sumbul roots (EOFS)
using GC-MS analysis and further evaluates mechanism-based anxiolytic potential of oil.
Materials and Methods:
EOFS was extracted using Clevenger apparatus, and was screened for anxiolytic
activity using an elevated plus maze model. A battery of models was subsequently used to
confirm the anxiolytic potential of EOFS. Further, benzodiazepine (BZD) receptor antagonist flumazenil
and pentylenetetrazole (PTZ) were used for investigating the possible involvement of GABA
receptors.
Results:
GC-MS analysis of EOFS revealed the presence of 32 components comprising triterpenoids
and their derivatives. The oil exhibited significant anxiolytic activity at 50 μl/kg in various models
like the elevated plus maze, light/dark, mirror chamber, open-field and mCPP-induced anxiety. The
observation that anxiolytic effect of EOFS was completely blocked by benzodiazepine (BZD) receptor
antagonist flumazenil, and partially by pentylenetetrazole, clearly demonstrates that anxiolytic
activity of the oil is mediated mainly through BZD site on GABA receptors. Further, a significant
reversal of mCPP induced anxiety by EOFS strongly indicates the possible involvement of 5-HT receptors
in mediating anxiolytic activity of the oil.
Conclusion:
Results of the present study clearly demonstrates the anxiolytic potential of EOFS and,
thus, validates the traditional relevance of the plant. This is the first report not only on multi-model
based antianxiety activity of EOFS but also on the possible antianxiety mechanism of the oil.
Funder
University Grants Commission
Publisher
Bentham Science Publishers Ltd.
Subject
Complementary and alternative medicine,Drug Discovery
Cited by
1 articles.
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