Affiliation:
1. Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida- 201309, India
Abstract
Background:
Microorganisms from understudied habitats have been shown to be an important
source of novel bioactive compounds. Endophytes constitute an underexplored group of microorganisms,
of which those from aquatic plants have been even less studied. Nelumbo nucifera
(lotus) is an aquatic plant with medicinal properties. A screening program for endophytes from N.
nucifera by our research group resulted in many microbial isolates, of which isolate L-003 was a
promising candidate, exhibiting antimicrobial and antioxidant activities.
Objectives:
The major objectives were to characterize the endophyte L-003 for its antimicrobial and
antioxidant properties, identify the constituent bioactive compounds by GC-MS and characterize
their activities further using in silico software.
Methods:
L-003 was identified by PIBWin software. Antimicrobial activity of the aqueous and organic
extracts of culture supernatant of L-003 was checked against a panel of bacteria and fungi.
Since the ethyl acetate extract showed the best antimicrobial activity, it was further characterized by
thin layer chromatography, an activity confirmed by bioautography and purified by column chromatography.
Total antioxidant capacity was assayed by standard techniques. Partially purified metabolite
fingerprints were identified by GC-MS analysis.
Results:
Based on morphological and biochemical analyses, isolate L-003 was identified as belonging
to Streptococcus sp. All the organic solvent extracts showed antimicrobial activity. Ethyl acetate
extract showed maximum antimicrobial activity against all selected targets and exhibited antioxidant
activity too, though butanol and aqueous extracts showed higher antioxidant activity. Two compounds,
Acetic acid,-hydroxy, methyl ester and Disulfide, dipropyl, were identified by GC-MS in
the metabolite fingerprint. These compounds showed differences in observed and calculated retention
indices and could, therefore, be novel. In silico activity, characterization confirmed the antimicrobial
and antioxidant properties attributed to these compounds.
Conclusion:
This is the first study reporting metabolite fingerprinting, identification and characterization
of bioactive compounds from an endophytic isolate of Nelumbo nucifera. We conclude that
endophytes from aquatic plants could be prospective sources of bioactive compounds, in this case
with antimicrobial and antioxidant activities.
Funder
Indian Council of Medical Research, India
Publisher
Bentham Science Publishers Ltd.
Subject
Complementary and alternative medicine,Drug Discovery
Reference24 articles.
1. Christina A.; Varghese C.; Bhore S.J.; Endophytic bacteria as a source of novel antibiotics: An overview. Pharm Rev 2013,7(13),11-16
2. Santoyo G.; Moreno-Hagelsieb G.; Orozco-Mosqueda M.; Del C.; Glick B. R.; Plant growth-promoting bacterial endophytes. Microbiol
Res 2016,183,92-99
3. Strobel G.; Daisy B.; Bioprospecting for microbial endophytes and their Natural Products. Microbiol Mol Biol Rev 2003,67(4),491-502
4. Ijarwal T.; Sharma B.; Khan F.; Ibeyaima A.; Dwivedi A.; Saini N.; Sarethy I.P.; Endophytes from the aquatic plant Nelumbo nucifera: Diversity profile and activity characterization. Int J Pharm Pharm Sci 2016,8(1),266-270
5. Bryant T.N.; PIBWin software for probabilistic identification. J Appl Microbiol 2004,97(6),1326-1327
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献