Affiliation:
1. Department of Clinical Pharmacy and Therapeutics, Applied Science Private University, Amman 11931-166, Jordan
Abstract
Background:
The activity of phytochemicals has a huge impact on pharmaceutical research
and developing new drugs. Hence, numerous studies have been conducted to investigate the efficacy
of different natural products and their potential to be used in different types of therapy.
Objective:
This study was carried out to investigate the biological activity of M. autumnalis crude
extract and fractions, and the assessment included antimicrobial activity, radical scavenging potential,
and their effect on the immune system. The work also emphasizes determining the chemical constituents
and the main bioactive compounds in M. autumnalis leaves.
Methods:
Ethanol crude extract was prepared by maceration method, followed by solvents fractionation
using n-hexane, aqueous/methanol, and water. Phytochemical analysis of ethanol extract using
LC-MS and total phenolic and flavonoid content were conducted. The immunomodulatory activity
was also evaluated by measuring the effect of M. autumnalis on splenocyte proliferation in the presence
and absence of mitogens. Nitro blue tetrazolium assay and neutral red method have been used to
determine the effect of M. autumnalis on the phagocytic activity of macrophages and the function of
pinocytosis, respectively. Antibacterial assay was applied using the microtiter plate dilution method,
and DPPH assay was used to determine the antioxidant activity.
Results:
The phytochemical analysis has shown the presence of flavonoids, polyphenolic compounds,
and other organic acids like quinic and chlorogenic acids. M. autumnalis aqueous fraction exhibited
high antioxidant activity with an IC₅₀ value of 23.67±0.3 μg/ml. Ethanol extract and n-hexane fraction
inhibited the bacterial growth of P. auriginosa with an MIC value of 25 mg/ml, while n-hexane fraction
was more active against C. albicans with an MIC value of 12.5 mg/ml. On the other hand, the
immune assay revealed a slight modulation of the lymphocyte and macrophage activity compared to
the control results.
Conclusion:
The results suggested that M. autumnalis leaves are rich in polyphenolic compounds and
its crude extract and fractions exhibited antimicrobial and antioxidant potential. However, further research
is needed to understand the effect of M. autumnalis on the immune parameters.
Publisher
Bentham Science Publishers Ltd.
Subject
Complementary and alternative medicine,Drug Discovery
Cited by
1 articles.
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