Affiliation:
1. Department of Applied Biotechnology, Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), Cau Giay, Hanoi, Vietnam
Abstract
Background::
Helicteres hirsuta has been used traditionally as a useful agent for hepatoprotective
treatment. The aim of the current study is to isolate chemical constituents from the
EtOAc extract of H. hirsuta stem and evaluate the capacity of the isolated compounds against hydroperoxide
damaged rat liver cells.
Methods::
Column chromatography was used for phytochemical isolation whereas MTT method was applied for intracellular antioxidative assay
Results::
Phytochemical analysis of the EtOAc extract of H. hirsuta stem led to the isolation and determination
of four triterpenoids betulin (1), bentulinic acid (2), alphitolic acid (3), and oleanolic
acid (4), together with two steroids stigmast-4-ene-6β-ol-3-one (5), and β-sitostenone (6), whereas
EtOAc extract of its leaf was composed of three steroids cucurbitacin D (11), cucurbitacin I (12),
and simiarenol (13), four flavonoids tiliroside (14), potengriffioside A (15), kaempferide (16), and
isokaempferide (17), along with two carotenoids lutelin (18), and β-carotene (19). Isolated compounds
3, 5, 6, 16, and 17 were found in genus Helicteres for the first time, while carotenoids 18,
and 19 were never isolated from family Sterculiaceae before. Phytochemicals derived H. hirsuta
species are also useful agents for antioxidative drugs, e.g, flavonol 17 induced the significant EC50
value of 22.24 ± 0.14 μg/mL, as compared with that of positive control curcumin (EC50 19.33 ±
0.77 μg/mL), against hydroperoxide damaged rat liver cells.
Conclusion::
Antioxidative activity of the EtOAc extract of H. hirsuta stem against hydroperoxide
is mostly based on the role of flavonoids.
Publisher
Bentham Science Publishers Ltd.
Subject
Complementary and alternative medicine,Drug Discovery
Cited by
2 articles.
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