Accumulation of Heavy Metals in Sepia officinalis Extract Aggravate Acute Kidney Injury Induced by a High Folic Acid Dosage in Wistar Rats

Author:

Abdel-Fattah Ahmed Salma Hussein1,El-Sayed El-Shehry Mona Samir Fawzy2,Mohamed Lotfy Bassant Mohamed2,Qutb Sarah Ali3,Rashed Aya Ramadan3,Mohamed Ayman Saber3

Affiliation:

1. Department of Biotechnology, Faculty of Biotechnology, October University for Modern Sciences and Arts (MSA), Egypt

2. Department of Biotechnology, Faculty of Biotechnology, October University for Modern Sciences and Arts (MSA), Egypt

3. Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt

Abstract

Background: Seafood is an important source of food for the majority of people. Marine species have a wide spectrum of pharmacological actions, including antibacterial, antiviral, antiparasitic, anti-inflammatory, and anti-diabetic properties. Objective: The purpose of this study was to examine the effects of Sepia officinalis extract (SoE) on folic acid-induced acute kidney injury in Wistar rats. Methods: A single dosage of folic acid (250 mg/kg) was injected intraperitoneally to cause kidney injury induced (AKI). The study contained three groups of six rats each: control, folic acid, and folic acid + SoE groups. The SoE group received SoE (45 mg/kg, orally) daily for one week, while the control and folic acid groups were administered distilled water. Results: The crude extract of Sepia officianlis contains heavy metals such as Fe, Cr, Cd, Pb, and Zn, according to our findings. The LD50 value of SoE was 450 mg/kg. SoE treatment increases creatinine, urea, uric acid, sodium, potassium, chloride, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyltransferase, malondialdehyde, and nitric oxide levels while decreasing total proteins, albumin, glutathione reduced, glutathione-S-transferase, and catalase. Several histological alterations were found in the liver and kidney of the SoE rats. Conclusion: The heavy metal content of S. officinalis extract has a synergistic effect with folic acid to induce hepatorenal injury. Natural extracts of marine species should be used with caution as a component of medications or natural remedies.

Publisher

Bentham Science Publishers Ltd.

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