Affiliation:
1. College of Horticulture and Gardening, Yangtze University, Jingzhou, Hubei Province 434025, China
2. Program of Cellular and Molecular Biology, Duke University, Durham, NC 27708, United States
Abstract
Background:
MicroRNAs participate in many molecular mechanisms and signaling transduction
pathways that are associated with plant stress tolerance by repressing expression of their target genes. However,
how microRNAs enhance tolerance to low temperature stress in plant cells remains elusive.
Objective:
In this investigation, we demonstrated that overexpression of the rice microRNA528 (OsmiR528)
increases cell viability, growth rate, antioxidants content, ascorbate peroxidase (APOX) activity,
and superoxide dismutase (SOD) activity and decreases ion leakage rate and thiobarbituric acid
reactive substances (TBARS) under low temperature stress in Arabidopsis (Arabidopsis thaliana),
pine (Pinus elliottii), and rice (Oryza sativa).
Methods:
To investigate the potential mechanism of OsmiR528 in increasing cold stress tolerance, we
examined expression of stress-associated MYB transcription factors OsGAMYB-like1, OsMYBS3,
OsMYB4, OsMYB3R-2, OsMYB5, OsMYB59, OsMYB30, OsMYB1R, and OsMYB20 in rice cells
by qRT-PCR.
Results:
Our experiments demonstrated that OsmiR528 decreases expression of transcription factor
OsMYB30 by targeting a F-box domain containing protein gene (Os06g06050), which is a positive
regulator of OsMYB30. In OsmiR528 transgenic rice, reduced OsMYB30 expression results in increased
expression of BMY genes OsBMY2, OsBMY6, and OsBMY10. The transcript levels of the
OsBMY2, OsBMY6, and OsBMY10 were elevated by OsMYB30 knockdown, but decreased by Os-
MYB30 overexpression in OsmiR528 transgenic cell lines, suggesting that OsmiR528 increases low
temperature tolerance by modulating expression of stress response-related transcription factor.
Conclusion:
Our experiments provide novel information in increasing our understanding in molecular
mechanisms of microRNAs-associated low temperature tolerance and are valuable in plant molecular
breeding from monocotyledonous, dicotyledonous, and gymnosperm plants.
Funder
National Natural Science Foundation of China
Publisher
Bentham Science Publishers Ltd.
Subject
Genetics(clinical),Genetics
Reference70 articles.
1. Devi SJ, Madhav MS, Kumar GR, Goel AK, Umakanth B, Jahnavi B, Viraktamath BC. Identification of abiotic stress miRNA Transcription Factor Binding Motifs (TFBMs) in rice.
2. Yang Y, Zhang X, Chen Y, Guo J, Ling H, Gao S, Su Y, Que Y, Xu L. Selection of reference genes for normalization of microRNA expression by RT-qPCR in sugarcane buds under cold stress.
3. Maeda S, Sakazono S, Masuko-Suzuki H, Taguchi M, Yamamura K, Nagano K, Endo T, Saeki K, Osaka M, Nabemoto M, Ito K, Kudo T, Kobayashi M, Kawagishi M, Fujita K, Nanjo H, Shindo T, Yano K, Suzuki G, Suwabe K, Watanabe M. Comparative analysis of microRNA profiles of rice anthers between cool-sensitive and cool-tolerant cultivars under cool-temperature stress.
4. Bredow M, Vanderbeld B, Walker VK. Knockdown of ice-binding proteins in demonstrates their role in freeze protection.
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