Affiliation:
1. Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target and Clinical Pharmacology,
The NMPA & State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & the Fifth Affiliated
Hospital, Guangzhou Medical University, Guangzhou, 511436, China
Abstract
Background:
Cancer is a serious disease severely endangering human health and life in
the world in the world, and conventional anticancer drugs have frequently suffered from severe side
effects and resistance. Therefore, developing novel and effective therapeutic strategies is urgently
needed. As a Chinese herbal medicine with important medical values, Hedyotis chrysotricha has
been suggested to be useful for the treatment of many diseases, especially cancer.
Objective:
Our aim was to investigate the antioxidant and antitumor activities of the phytochemical
extracts of H. chrysotricha.
Methods:
2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay was applied to determine
antioxidant property. 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay, wound
healing assay, transwell matrix assay, flow cytometry and cell morphology were established to observe
anti-proliferative, anti-migration, anti-invasion and apoptosis induction effects. The intracellular
reactive oxygen species generation was detected by reactive oxygen species detection assay.
Results:
The ethyl acetate fraction showed promising antioxidant activity with IC50 of 98.67±0.49
μg/ml, and petroleum ether fraction exhibited the most potent antiproliferative effect on various human
cancer cell lines, especially MDA-MB-231 (IC50 = 8.90±1.23 μg/ml) and HCT-116 (IC50 =
9.69±3.69 μg/ml) cancer cells. Further investigation revealed that the petroleum ether fraction suppressed
the proliferation, migration and invasion of the cancer cells significantly. Additionally, it
promoted cell apoptosis by increasing reactive oxygen species levels.
Conclusion:
H. chrysotricha extract possessed excellent antioxidant and antitumor activities. Therefore,
it could be useful as a source of antioxidants and compounds for cancer therapy.
Funder
Natural Science Foundation of Guangdong Province
Special Fund for Guangdong Province's Science and Technology Innovation Strategy
Publisher
Bentham Science Publishers Ltd.