Affiliation:
1. Department of Clinical Pharmacy and Therapeutic, Applied Science Private University, Amman 11931-166, Jordan
Abstract
Background:
Probiotics exhibit significant activity in the immune system by activating
multiple immune mechanisms. Resveratrol is a non-flavonoid polyphenol. It has various pharmacological
effects, including anti-oxidative, anti-inflammatory, anti-proliferative, and anti-angiogenesis
effects.
Objectives:
The present study investigates the potential immunomodulatory and antitumor activity
of the combination treatment of probiotics and resveratrol in sensitive and cisplatin-resistant breast
cancer.
Methods:
In the in vivo study, tumor-bearing mice received one of the following treatments:
2.5*108 CFU/ml probiotics, 50 mg/kg resveratrol, the combination of probiotics and resveratrol,
vehicle, or cisplatin. Balb/C mice were inoculated with sensitive EMT6/P and cisplatin resistance
EMT6/CPR cancer cell lines, and in vivo antitumor activity was evaluated.
Results:
The antiproliferative activity of the probiotics, resveratrol, and their combination treatments
was assessed using an MTT assay to evaluate lymphocyte proliferation activity. LDH colorimetric
assay was conducted to measure the effectiveness of the treatments on the activity of natural
killer cells. Nitro blue tetrazolium assay and neutral red method were used to evaluate macrophage
function.
Conclusion:
The combination treatment showed an enhanced effect in splenic lymphocyte proliferation,
macrophage function, phagocytosis, and pinocytosis in both cell lines. A significant reduction
in tumor size and weight in EMT6/P and EMT6/CPR-bearing mice occurred. Hence, the combination
treatment of probiotics and resveratrol was found to have a valuable activity against sensitive
and cisplatin-resistant breast cancer cells and might act as a stimulator of the immune system.
Therefore, the combination of probiotics and resveratrol deserves further analysis to be used in
cancer prevention and treatment.
Funder
Applied Science Private University, Amman, Jordan
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmacology (medical),Endocrinology