Affiliation:
1. Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China
2. The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases, The First Affiliated Hospital of Chongqing
Medical University, Chongqing, 400016, China
Abstract
Background:
Breast cancer is the most frequently diagnosed malignancy and the leading cause of cancerrelated
deaths in women. Activation of EGFR by EC-secreted EGFR ligands promotes breast cancer progression. Current
treatments provide limited benefits in triple-negative breast cancer (TNBC). Photodynamic therapy (PDT) has
been proven effective for the treatment of TNBC through the EGFR pathway, but the underlying mechanism is still
unclear.
Purpose:
The purpose of this study was to determine the role of the EGFR pathway in the treatment of PDT on TNBC
in a co-culture system.
Methods:
MB-231 and HUVEC were co-cultured for experiments (HU-231). Cell viability and ROS production were
detected after AE-PDT, a combination of EGFR inhibitors (AEE788)with PDT to test angiogenesis, apoptosis, and
pyroptosis. WB detects expression of EGFR. EGFR, P-EGFR, VEGF, caspase-1, capase-3, and GSDMD .
Results:
AE-PDT inhibited HU-231 cell proliferation and tumor angiogenesis, and induced cell apoptosis and pyroptosis
by promoting ROS production. AEE788, an inhibitor of the EGFR, enhanced HU-231 cell killing after AE-PDT
Conclusion:
Our study suggested that the combination of EGFR inhibitors and AE-PDT could synergistically suppress
breast cancer progression, providing a new treatment strategy.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Chongqing
Cultivating Fund in the First Affiliated Hospital of Chongqing Medical University
Publisher
Bentham Science Publishers Ltd.
Subject
Cancer Research,Pharmacology,Molecular Medicine
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