NF-κB1 Intronic Region Polymorphisms as Risk Factor for Head and Neck Cancer in HPV-Infected Population from Pakistan
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Published:2022-01
Issue:1
Volume:22
Page:74-82
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ISSN:1566-5240
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Container-title:Current Molecular Medicine
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language:en
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Short-container-title:CMM
Author:
Sarwar Sumaira1,
Tareen Muammad Usman1,
Sabir Maimoona2ORCID,
Sultan Aneesa1,
Malik Salman A.1
Affiliation:
1. Department of Biochemistry, Quaid-i-Azam University, Islamabad, 75500, Pakistan
2. Department of Microbiology, University of Haripur, Khyber Pakhtunkhwa 75500, Pakistan
Abstract
Background:
Head and neck cancer (HNC) develops due to a number of risk
factors, including infection of Human Papillomavirus (HPV). The genetic predisposition
also plays an important role in deregulating different signaling pathways including the
NF-KB pathway. Certain polymorphisms are reported to affect the NF-kB pathway
genes.
Objectives:
The present research was conducted to study the association of HPV with
NF-KB1 (p50) gene polymorphisms in HNC patients of the Pakistani population.
Methods:
Genomic DNA from HNC tumors samples was extracted using the Exgene
SV DNA extraction Kit. Allele-specific PCR and direct sequencing were done for analysis
of NF-κB1 SNPs 94ins/del (rs28362491), rs1598858 and rs4648068.
Results:
The genotypes AGrs1598858, AGrs4648068 and GGrs4648068 were associated with
significantly increased risk of head and neck cancer in studied population. Furthermore
the HNC cases with genotypes AGrs1598858 and GGrs4648068 displayed growing risk of HPV
related cancers. Promotor region SNP 94ins/del (rs28362491) was not detected in
studied population. Tobacco use, lymph nodes involvement and poorly differentiated
tumors were positively associated with HPV induced cancers.
Conclusion:
It is the first comprehensive study from Pakistan, to evaluate the
polymorphic variants of NF-κB1. Genotypes AGrs4648068, GGrs4648068, and AGrs1598858 of
NF-κB1 gene are associated with increased risk of head and neck cancers in the studied
HPV infected Pakistani population. It can be concluded that HPV infection, involvement
of lymph nodes and tobacco use can act synergetic and add up in modulating HPV
induced HNC with intronic SNPs of NF-κB1 gene in Pakistani population.
Funder
Higher Education Commission of Pakistan
Publisher
Bentham Science Publishers Ltd.
Subject
Molecular Biology,Molecular Medicine,General Medicine,Biochemistry