Affiliation:
1. College of Human Kinesiology, Shenyang Sport University, 36 Jinqiansong East Road Sujiatun District, Shenyang, 110102, Liaoning,China
2. Department of basic medical, HE’s University, Shenyang, Liaoning 110163,China
3. College of clinical, HE’s University, Shenyang, Liaoning 110163,China
4. Experimental Teaching Center of Pharmacology, School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang , Liaoning 110016,China
Abstract
Aim:
The present study investigated whether melatonin (MEL) and enriched
environment (EE) might protect against intrauterine growth retardation (IUGR) in rats.
Methods:
Sprague-Dawley rats were randomly allocated to 3 groups: control (C), model
(M) and EE+MEL group. Animals were housed in an enriched environment (EE+MEL
group) or remained in a standard environment (C group, M group). IUGR rat model was
built by feeding a low protein diet during pregnancy. MEL was administered by gavaging.
At day 1 post-birth, the baseline characteristics and serum biochemical parameters,
morphology of liver and small intestine, enzyme activities, and mRNA expression levels
of fetal rats were determined. The autophagy marker LC3 and Beclin1 were determined
by western blot analysis.
Results:
EE+MEL markedly improved the baseline characteristics, hepatic and intestinal
morphology of IUGR fetuses. In addition, the lactase activities in the fetal intestine were
markedly increased by the EE+MEL. The levels of serum somatostatin (SST), Growth
hormone (GH), GH releasing hormone (GHRH), Insulin-like Growth Factor 1 (IGF-1),
triiodothyronine (T3), and tetraiodothyronine (T4) were found to be recovered by
EE+MEL. In addition, the EE+MEL significantly ameliorated the mRNA expression of
SST, GHRH, and GHRH receptor (GHRHR), GH, GHR, IGF-1, and IGF-1 receptor
(IGF1R), IGF binding protein-1 (IGFBP1), mammalian target of rapamycin (mTOR), S6
kinase 1 (S6K1) and eukaryotic initiation factor 4E (eIF4E)-binding protein 1 (4EBP1) in
fetuses. In IUGR fetal livers, LC3 and Beclin1 were found to be increased at birth, while
LC3 and Beclin1 were observed to be significantly decreased in the EE+MEL group.
Conclusion:
EE+MEL could improve fetal rats' baseline characteristics, serum
biochemical parameters, birth weight, intestinal and hepatic morphology and enzyme
activities. These effects could be explained by the activation of the IGF-1/IGFBP1 and
IGF-1/mTOR/S6K1/4EBP1 signaling pathway and autophagy inhibition.
Publisher
Bentham Science Publishers Ltd.
Subject
Molecular Biology,Molecular Medicine,General Medicine,Biochemistry
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