Affiliation:
1. Department of Chemical and Product Safety, German Federal Institute for Risk Assessment (BfR), Max-Dohrn-Strasse 8-10, 10589 Berlin,Germany
Abstract
:
Compared to the classical chemicals, nanoparticles (NPs) exhibit unique properties,
which lead to challenges in sample preparation and analysis. Fractionation techniques
and, in particular, hollow fiber flow field flow fractionation (HF5) have recently
become popular in the characterization and quantification of nanomaterials, because of
their fine fractionation capability in the nanoscale-range. When dealing with NPs, a great
drawback during fractionation is the loss of particles in the fractionation devices, tubing
and connectors. There is a need for studies to systematically explore and assess the quality
of the fractionation process. A combination of two complementary mass-based setups
was used to determine particle loss in HF5. Inductively coupled plasma mass spectrometry
(ICP-MS) enabled the estimation of recovery rates for NPs after HF5 separation. Reciprocally,
laser ablation ICP-MS (LA-ICP-MS) permitted the evaluation of particles retained
on the hollow fiber. 15 nm Au-NPs in different concentrations were evaluated in
this study and showed a recovery level for Au-NPs of 50 – 65% based on the applied concentrations
after a complete HF5 separation run. Detection of sample deposition on the
hollow fiber by LA-ICP-MS indicated a sample loss of about 8%. These findings are important
for experiments relying on fractionation of low concentrated nanoparticulate samples.
Funder
German Federal Institute of Risk Assessment
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmacology,Molecular Medicine,Drug Discovery,Biochemistry,Organic Chemistry
Cited by
4 articles.
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