Affiliation:
1. Federal Research Centre for Cultivated Plants, Institute for Biosafety in Plant Biotechnology, Erwin-Baur- Str. 27, D-06484, Quedlinburg, Germany
Abstract
During the last two decades, the production of pharmaceutical proteins in plants
evolved from proof of concept to established technology adopted by several biotechnological
companies. This progress is particularly based on intensive research starting stable genetic
transformation and moving to transient expression. Due to its advantages in yield and
speed of protein production transient expression platforms became the leading plant-based
manufacturing technology. Current transient expression methods rely on Agrobacteriummediated
delivery of expression vectors into plant cells. In recent years, great advances have
been made in the improvement of expression vectors, host cell engineering as well as in the
development of commercial manufacturing processes. Several GMP-certified large-scale
production facilities exist around the world to utilize agroinfiltration method. A number of
pharmaceutical proteins produced by transient expression are currently in clinical development.
The great potential of transient expression platform in respect to rapid response to
emerging pandemics was demonstrated by the production of experimental ZMapp antibodies
against Ebola virus as well as influenza vaccines. This review is focused on current design,
status and future perspectives of plant transient expression system for the production
of biopharmaceutical proteins.
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmacology,Molecular Medicine,Drug Discovery,Biochemistry,Organic Chemistry
Cited by
40 articles.
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