Effect of Intestinal Flora Clearance on Liver Proteomics in Mice
Author:
Jia Zhenghu1, Liu Hui1, Song Mei1, Yang Chengmao1, Zhao Yapu2, Wu Xiaoli3, Wu Zhenzhou1, Zhao Liqing1
Affiliation:
1. State Key Laboratory of Medicinal Chemical Biology (Nankai University), College of Life Sciences, Nankai University, Tianjin, 300071, China 2. People's Liberation Army No. 254 Hospital, Tianjin, 300142, China 3. School of Life Sciences, Tianjin University, Tianjin Engineering Center of Micro Nano Biomaterials and Detection Treatment Technology, and Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, 300072, China
Abstract
Background:
Intestinal flora dynamically affects the host's systemic immune system. Liver
is one of the organs that may be affected by intestinal microbiota.
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Materials and Methods: In this study, we aimed to identify proteome level differences between liver
tissue from mice cleared intestinal flora and control using tandem mass spectrometry (LC-MS/MS) and
label free quantification. Additionally, protein-protein interactions were mapped by STRING, and also,
the enrichment of inflammation-related signaling pathways and biological processes was identified using
GO and IPA network system. RT-PCR and Western blot were used for validation of the proteomics
findings.
Results:
Our study demonstrated that mice with cleared intestinal flora exhibited decreased sensitivity
to Concanavalin A induced acute hepatitis. 324 Proteins in liver were differently expressed after intestinal
flora clearance for one week while 210 proteins were differently expressed after intestinal flora
clearance for two weeks. Furthermore, five of the identified proteins were validated by western blotting
and further investigated by semi-quantitative RT-PCR.
Conclusion:
Our results showed that intestinal flora clearance in mice could reduce sensitivity to Concanavalin
A induced liver injury and influence the expression of proteins in liver, which provides a
clue for studying the relationship between gut bacteria and Concanavalin A induced hepatitis.
Funder
Natural Science Foundation of State Key Laboratory of Medicinal Chemical Biology, Nankai University Natural Science Foundation of Tianjin City
Publisher
Bentham Science Publishers Ltd.
Subject
Molecular Biology,Biochemistry
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