The Combination of the CIGB-300 Anticancer Peptide and Cisplatin Modulates Proteins Related to Cell Survival, DNA Repair and Metastasis in a Lung Cancer Cell Line Model
Author:
Rodríguez-Ulloa Arielis1, Ramos Yassel1, Sánchez-Puente Aniel1, Perera Yasser2, Musacchio-Lasa Alexis3, Fernández-de-Cossio Jorge3, Padrón Gabriel1, López Luis J.G.1, Besada Vladimir1, Perea Silvio E.2
Affiliation:
1. Department of Proteomics, Center for Genetic Engineering and Biotechnology, Havana, Cuba 2. Laboratory of Molecular Oncology, Center for Genetic Engineering and Biotechnology, Havana, Cuba 3. Department of Bioinformatics, Center for Genetic Engineering and Biotechnology, Havana, Cuba
Abstract
Background:
CIGB-300 is a pro-apoptotic peptide that abrogates CK2-mediated phosphorylation,
and can elicit synergistic interaction in vitro and in vivo when combined with certain anticancer
drugs.
Objective:
The combination of CIGB-300 with cisplatin is studied through data mining and expressionbased
proteomics to reveal the molecular basis of this interaction. Cisplatin resistance-associated proteins,
which have also been reported as CK2 substrates, were first identified by bioinformatic analyses.
Methods:
Data from these analyses suggested that the cisplatin resistance phenotype could be directly
improved by inhibiting CK2 phosphorylation on specific substrates. Furthermore, 157 proteins were
differentially modulated on the NCI-H125 lung cancer cell line in response to CIGB-300, cisplatin or
both drugs as determined by LC-MS/MS.
Results:
The expression of 28 cisplatin resistance-associated proteins was changed when cisplatin was
combined with CIGB-300. Overall, the proteins identified are also related to cell survival, cell proliferation
and metastasis. Furthermore, the CIGB-300 regulated proteome revealed proteins that were initially
involved in the mechanism of action of CIGB-300 and cisplatin as single agents.
Conclusion:
This is the first report describing the protein array modulated by combining CIGB-300
and cisplatin that will support the rationale for future clinical settings based on a multi-target cancer
therapy.
Publisher
Bentham Science Publishers Ltd.
Subject
Molecular Biology,Biochemistry
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