Affiliation:
1. Capital University of Science and Technology, Islamabad, Pakistan
Abstract
Aims:
To find out Potential Drug targets against HPV E7.
Background:
Oncoprotein E7 of Human Papilloma Virus (HPV-16), after invading human body
alter host protein-protein interaction networks caused by the fluctuations of amino acid residues present
in E7. E7 interacts with Rb protein of human host with variable residual fluctuations, leading
towards the progression of cervical cancer.
Objective:
Our study was focused our computational analysis of the binding and competing interactions
of the E7 protein of HPV with Rb protein.
Methods:
Our study is based on analysis of dynamic fluctuations of E7 in host cell and correlation
analysis of specific residue found in motif of LxCxE, that is the key region in stabilizing interaction
between E7 and Rb.
Results and Discussion:
Cysteine, Leucine and Glutamic acid have been identified as hot spot residues
of E7 which can provide platform for drug designing and understanding of pathogenesis of
cervical cancer, in future. Our study shows validation of the vitality of linear binding motifs LxCxE
of E7 of HPV in interacting with Rb as an important event in propagation of HPV in human cells
and transformation of infection into cervical cancer.
Conclusion:
Our study shows validation of the vitality of linear binding motifs LxCxE of E7 of
HPV in interacting with Rb as an important event in propagation of HPV in human cells and transformation
of infection into cervical cancer.
Other:
E7 interacts with Rb protein of human host with variable residual fluctuations, leading towards
the progression of cervical cancer.
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Pharmaceutical Science,Molecular Medicine