Affiliation:
1. Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, 201309, India
2. Research and Development Department, Decode DNA Pvt. Ltd., 16/2 Ashok Nagar, New Delhi, 110018, India
Abstract
Aims::
This study aimed at bioprospecting underexplored extreme habitats (Thar desert, India)
for novel bio- and chemo-diversity.
Background::
Bioactive metabolites from microorganisms, such as fungi from underexplored habitats,
serve as basic skeletons of therapeutic agents, including antimicrobials, combating the effect of multidrug
resistance of pathogens.
Objectives::
The main objectives of the current study are (i) characterization of isolate TD-082 and (ii)
metabolite fingerprinting of butanol extract showing antimicrobial compounds.
Methods::
In search of novel antimicrobial drugs, a promising microcolonial fungus TD-082, obtained
from the Thar Desert, India, was identified by ITS1–5.8S–ITS2 sequencing. Phenotypic characteristics
were marked by microscopy. The fungus was investigated for antimicrobial activity against a panel of
Gram-positive, Gram-negative bacteria and fungi. Butanol extract that showed the best antimicrobial
activity was partially purified; fractions exhibiting antimicrobial activity were pooled and fingerprinted by
GC-MS analysis.
Results::
Sequencing data indicated that the isolate belonged to Aureobasidium sp. It showed 96% similarity
to Aureobasidium iranianum and Kabatiella bupleuri, and 95 % to A. thailandense and A. subglaciale.
Microscopy results confirmed that it belongs to Aurebasidium sp. Metabolite fingerprinting showed tentatively
ten novel compounds belonging to three major categories, hydrocarbons, fatty acids, and peptides.
Conclusion::
The study shows that understudied habitats, such as deserts, can provide skeletons for novel
compounds from novel microorganisms. The study can be expanded to other niche habitats with higher
chances of identifying more novel bioactive compounds.
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Pharmaceutical Science,Molecular Medicine