The effects of Trigonella foenum-graecum L. on post-surgical adhesion band formation

Abstract

Background: Intra-abdominal adhesions are severe complications which occur after abdominal surgery. Currently, no specific anti-adhesive medications can completely prevent Intra-abdominal adhesion formation. Therefore, recent studies are exploring new approaches for preventing this complication. Anti-inflammatory properties of Trigonella foenum-graecum L. (Fenugreek) have been reported in various studies. In this experiment, a murine model was used to evaluate the potential anti-adhesive activity of Fenugreek in vivo. Objective: This experiment aimed to examine the anti-adhesive activity of Fenugreek in the prevention of postsurgical Intra-abdominal adhesions. Methods: We have adhered to the ARRIVE guidelines during these experimental studies. After abdominal surgery, for nine days, Fenugreek (400 mg/kg) was given by gavage to male Wistar rats (n=6). Following that, all animals were sacrificed to assess the anti-inflammatory and anti-fibrotic effects of Fenugreek using Hematoxylin & eosin staining and Masson’s trichrome staining. Results: Our results showed that Fenugreek hydro-alcoholic extract could significantly reduce the adhesion band formation based on Nari and Leach Scoring system (P < 0.01). The histological assessment also represented less inflammatory cell infiltration and less collagen deposition in the treatment group than in the positive control group (P < 0.01). Conclusion: This study showed that Fenugreek extract could attenuate post-surgical adhesion band formation by inhibiting pathological responses (Inflammation and fibrosis) following surgery.