Affiliation:
1. Department of Molecular Biology and Genetics, Kirsehir Ahi Evran University, TR-40100 Kirsehir, Turkey
2. Department of Genetics and Bioengineering, Kirsehir Ahi Evran University, TR-40100 Kirsehir, Turkey
3. Department of Plant
Protection, Kirsehir Ahi Evran University, TR-40100 Kirsehir, Turkey
Abstract
Background:
Annona muricata is a member of the Annonaceae family. This plant has
a high concentration of acetogenin, which gives it excellent therapeutic property. Researchers have
tested this miraculous herb in breast cancer cells treatment and observed that it could be a source
of anti-cancer agents. The proposed study focused on screening the anticancer biological activity
of Annona muricata plant by using the in vitro, in vivo, and in silico methods.
Methods:
In in vitro analysis, the IC50 was determined on two-dimensional and three-dimensional
breast cancer cells. 2D cells were cultured on flat dishes typically made of plastic, while 3D cells
were cultured using the hanging drop method. In in vivo analysis, Drosophila melanogaster was
preferred, and the LC50 was determined. In in silico analysis, molecular docking studies have been
carried out on the different classes of Annona muricata acetogenins against the target proteins.
Nearly, five acetogenins were selected from the literature, and docking was performed against human
Bcl-2, Bad and Akt-1 proteins.
Results:
In vitro and in vivo results revealed the IC50 value of 2D MDA-MB-231 cells as 330
μg.mℓ-1, of 2D MCF-7 cells as290 μg.mℓ-1, and of 3D MCF-7 and MDA-MB-231 cells about
0.005 g.mℓ-1; the LC50 value of Drosophila melanogaster was determined as 0.1 g.mℓ-1. In silico
results revealed that the docked complex formed by Isoquercetin showed better binding affinity
towards target proteins.
Conclusion:
As a result of the analysis, the Annona muricata plant has been observed to be effective
against cancer and likely to be a potential drug.
Funder
Kırsehir Ahi Evran University, Central Research and Application Laboratory
Publisher
Bentham Science Publishers Ltd.
Subject
Drug Discovery,Molecular Medicine,General Medicine