Pharmacokinetic Study of Deltaline in Mouse Blood Based on UPLCMS/ MS
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Published:2019-01-04
Issue:2
Volume:15
Page:194-199
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ISSN:1573-4129
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Container-title:Current Pharmaceutical Analysis
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language:en
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Short-container-title:CPA
Author:
Song Huanchun1, Huang Yiwei2, Zhu Dongqing3, Tong Shuhua1, Zhang Meiling2, Wang Xianqin2, Bao Xi3
Affiliation:
1. Department of Clinical Pharmacy, Jinhua Central Hospital, Jinhua 321000, China 2. Analytical and Testing Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China 3. The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
Abstract
Introduction: Deltaline, an aconitine-type alkaloid, was detected in mouse blood using an
ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method, and the
pharmacokinetics of deltaline following intravenous administration in mice was studied.
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Materials and Methods: The gelsenicine was used as the internal standard (IS). Deltaline and IS were
eluted at a flow rate of 0.4 ml/min and separated on a UPLC BEH C18 column by gradient elution using
acetonitrile and 10 mmol/L ammonium acetate (0.1% formic acid) as a mobile phase. The following
transitions were obtained at m/z 508.2→75.0 for deltaline and m/z 327.1→107.8 for gelsenicine in multiple
reactions monitoring mode. Acetonitrile was used to precipitate protein. Six mice after intravenous
administration of a single dose of deltaline (1 mg/kg), 20-µL blood samples from each mouse were
collected from the tail vein.
Results:
The UPLC-MS/MS method was sensitive and linear (r>0.995) with a lower limit of quantitation
(LLOQ) of 0.1 ng/mL over the range of 0.1-500 ng/mL. Intra- and inter-day precisions were below
13%, the accuracy range was between 88.0% and 108.2%, the recovery was higher than 90.1%, and the
matrix effect was between 102.9% and 108.1%.
Conclusion:
The method was sensitive, fast, specific, and has been successfully applied to a pharmacokinetic
study of deltaline after intravenous administration.
Funder
"Social Development of Jinhua City Bureau of Science and Technology Key Projects
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmaceutical Science,Molecular Medicine,Biochemistry,Biophysics
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