Determination of Menbutone Residues in Edible Swine Tissues Based on Solid-Phase Extraction and RP-HPLC

Author:

Zhou Liya1,Zhang Ping2,Liu Jiangbo1,Wu Xiaobo1,Luo Lei1,Xu Xingran1,Luo Yonghuang1

Affiliation:

1. College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China

2. Chongqing Municipal Agriculture School, Chongqing 401329, China

Abstract

Background: Menbutone is widely used as a veterinary choleretic drug in many countries. There was no publicly available analysis method for the determination of menbutone residues in swine tissues. It is necessary to establish a method to control the maximum residue limit and ensure food safety of the public. Objective: The aim of this study is to establish an analytical method for the simultaneous determination of menbutone in muscle, fat, liver and kidney tissues from swine. Methods: MBT residue was extracted by acetonitrile from the tissues then purified by using a C18 solid phase extraction (SPE) cartridge and an alkaline alumina (ALA) SPE cartridge. MBT was detected by RP-HPLC and separation was achieved on a Shim-pack VP-ODS C18 HPLC column using phosphoric acid solution (0.5%, v/v) and acetonitrile (45/55, v/v) at a flow rate of 1.0 mL/min. The effluent was monitored at 235 nm, and the column temperature was set to 30°C. Results: MBT eluted at 6.3 min and no interfering peak nearby was observed. This linearity within the concentration range of 0.02 (LOQ) and 12 µg/mL (r2>0.9999, n=6). The accuracy ranged from 74.07 to 110.83% of the actual values. Intra and inter-day precision were within 15.11%. In the application study, MBT was detectable in continuously dosing MBT 10 µg/g/day to healthy swine for 7 days. Conclusion: The proposed method has specificity, accuracy, and sensitivity, with an excellent linear relationship that successfully applied to swine tissues.

Funder

Chongqing Science and Technology Commission

National Natural Science Foundation of China

National key research and development program of China

Publisher

Bentham Science Publishers Ltd.

Subject

Pharmaceutical Science,Molecular Medicine,Biochemistry,Biophysics

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