Affiliation:
1. College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China
2. Chongqing Municipal Agriculture School, Chongqing 401329, China
Abstract
Background:
Menbutone is widely used as a veterinary choleretic drug in many countries.
There was no publicly available analysis method for the determination of menbutone residues in swine
tissues. It is necessary to establish a method to control the maximum residue limit and ensure food
safety of the public.
Objective:
The aim of this study is to establish an analytical method for the simultaneous determination
of menbutone in muscle, fat, liver and kidney tissues from swine.
Methods:
MBT residue was extracted by acetonitrile from the tissues then purified by using a C18 solid
phase extraction (SPE) cartridge and an alkaline alumina (ALA) SPE cartridge. MBT was detected by
RP-HPLC and separation was achieved on a Shim-pack VP-ODS C18 HPLC column using phosphoric
acid solution (0.5%, v/v) and acetonitrile (45/55, v/v) at a flow rate of 1.0 mL/min. The effluent was
monitored at 235 nm, and the column temperature was set to 30°C.
Results:
MBT eluted at 6.3 min and no interfering peak nearby was observed. This linearity within the
concentration range of 0.02 (LOQ) and 12 µg/mL (r2>0.9999, n=6). The accuracy ranged from 74.07 to
110.83% of the actual values. Intra and inter-day precision were within 15.11%. In the application
study, MBT was detectable in continuously dosing MBT 10 µg/g/day to healthy swine for 7 days.
Conclusion:
The proposed method has specificity, accuracy, and sensitivity, with an excellent linear
relationship that successfully applied to swine tissues.
Funder
Chongqing Science and Technology Commission
National Natural Science Foundation of China
National key research and development program of China
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmaceutical Science,Molecular Medicine,Biochemistry,Biophysics
Reference28 articles.
1. Lund J.; Lassen J.B.; Elimination and distribution of menbutone (Genabil®) in rats. Basic Clin Pharmacol 1969,27,429-438
2. The European Agency for the Evaluation of Medicinal Products: Committee for veterinary medicinal products menbutone summary report (Accessed on: June 5, 2018
3. Symonds H.; The choleretic effect of menbutone and clanobutin sodium in steers. Vet Rec 1982,110,423-425
4. Larska M.; Krzysiak M.; Smreczak M.; Polak M.P.; Żmudziński J.F.; First detection of Schmallenberg virus in elk (Alces alces) indicating infection of wildlife in białowieża National Park in Poland. Vet J 2013,198,279-281
5. Okada K.; Kikuchi K.; Miura K.; Sato T.; Morita Y.; Tako M.; Ogino T.; Kaneda Y.; Relationship between white scour of calf and alcohol stability of milk in Japanese black cattle. J Jpn VetMed Assoc, 1997,50,74-79
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献