Identification of Methamphetamine and Amphetamine in Drug Abuse Urine Sample using Multi-drug Rapid Test and Gas Chromatography- Mass Spectrometry (GC-MS) at the Centre of Drug Testing Laboratory, National Narcotics Board Republic of Indonesia

Abstract

Introduction: Drug abuse is a real threat that threatens the Indonesian nation in the form of non-military threats. The second most abused illicit substance in the world is methamphetamine (MA). Most of the methamphetamine users in the world live in East and Southeast Asia. Methamphetamine is a powerful stimulant of the central nervous system (CNS) that can make someone feel an increase in sympathomimetic. Methods: First, the preliminary test was conducted using a BioCare multi-drug rapid test and a specific methamphetamine rapid test that works based on the Lateral Flow Assay (LFA) principle. Second, the sample preparation used Liquid-Liquid Extraction (LLE). Third, GC-MS confirmed the test using GCMS Shimadzu-QP2020NX-GC-2030 (HP-5MS column, Length 30 m, diameter 0.25 m, film 0.25). Result and Discussion: The Preliminary test shows that the urine is positive and contains methamphetamine and amphetamine. Based on the sample chromatogram, There are 2 peaks, which are methamphetamine and amphetamine compounds it can be seen by the retention time is 5.560 minutes (methamphetamine) a similarity index of 98% to methamphetamine compounds, and 4.885 minutes (amphetamine) with a similarity index of 96% to amphetamine compounds. Conclusion: The urine samples with code 210DGB from methamphetamine users were detected due to the presence of methamphetamine and amphetamine, which is the metabolite of methamphetamine.