Affiliation:
1. Laboratory Animal Centre, Wenzhou Medical University, Wenzhou 325035, China
2. Analytical and Testing Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325000, China
3. The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
Abstract
Background:
Xuezhikang capsule, which contains cholesterol synthase inhibitors and a
large number of natural statins, is put in the clinical application of lipid-lowering and so on.
However, the specific use of dose, lipid-lowering effect and the relationship between metabolites
are to be further studied.
Introduction:
Metabonomics is the study of the relationship between the change of quantity and
physiological changes from metabolites. At present metabolomics has been widely used in drug
development and testing. In this study, we developed a metabolomic method based on gas
chromatography-mass spectrometry (GC-MS) to find out hyperlipemia-related substances, and
study the lipid-lowering mechanism of Xuezhikang.
Method:
Fifty SD rats (220 ± 20 g) were given high-fat diet. After four-weeks modeling, they
were randomly divided into semi-control group, high fat group, simvastatin intervention group and
Xuezhikang intervention group (0.23, 0.69, 1.15 mg/kg, low, medium, high), each dosage in eight
rats. The control group (rest eight rats) were given normal diet, and no specific treatment. The rats
were sacrificed at the end of the experiment.
Result:
The biochemical and body weight indexes of the normal control group and the high fat
group were significantly different (P <0.05), which indicated that the model of hyperlipidemia was
established success. There was significant difference (P <0.05) between Xuezhikang intervention
group and high fat control group (P <0.05), and hyperlipemia metabolomics related markers,
oxalic acid, butyric acid, mannitol, glucose, glucuronic acid were found. Glucuronic acid and
non-binding bilirubin combined with bilirubin, combined with some of the liver harmful
substances, play a detoxification effect.
Conclusion:
The results of metabonomics showed that the high-fat group and the control group have
significant differences. Mannose, glucose content is relatively stable, lipid metabolism in high-fat
group stearic acid, palmitic acid levels decreased, suggesting that high-fat diet disorders rat body lipid
metabolism. It is worth mentioning that the experimental evaluation of rats, such as biochemical indicators
and pathological results are prompted to model success, Xuezhikang intervention effect is more
significant, consistent with the expected.
Conclusion:
The results of metabonomics showed that the high fat group and the control group
were significant difference. Mannose, glucose content is relatively stable, lipid metabolism in
high-fat group stearic acid, palmitic acid levels decreased, suggesting that high-fat diet disorders
rat body lipid metabolism. It is worth mentioning that the experimental evaluation of rats such as
biochemical indicators and pathological results are prompted to model success, Xuezhikang
intervention effect is more significant, consistent with the expected.
Funder
Zhejiang Provincial Natural Science Foundation of China
Medical and Health Research Project of Zhejiang Province
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmaceutical Science,Molecular Medicine,Biochemistry,Biophysics
Cited by
1 articles.
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