Affiliation:
1. Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, AP, 522510, India | Department
of Chemistry, Sir CR Reddy College, Eluru, AP, 534007, India
2. Department of Chemistry, Vignan Degree College,
Guntur, AP, 522009, India
3. Department of Chemistry, Vignans Nirula Institute of Technology and Science for Women,
Guntur, AP, 522009, India
Abstract
Background:
Verapamil is an excellent drug used for the medication of hypertension
and trandolapril. It is an angiotensin-converting-enzyme inhibitor. Hence, it is an interesting
method to develop a novel and reliable MS/UPLC strategy for the simultaneous development of
verapamil and trandolapril.
Objective:
This research study aims to develop a new, rapid, and sensitive UPLC-MS/MS method
for the simultaneous estimation of verapamil and trandolapril in rat plasma using D6-
verapamil and D6-trandolapril.
Method:
Separation was carried on column Symmetry C18 column (150x4.6 mm, 3.5 μm) using
isocratic elution with a buffer containing 1mL of formic acid in 1L of water and the mixture of
two components like Buffer and Acetonitrile in the ratio of 80:20 as mobile phase with
1mL/min flow rate at ambient temperature.
Results:
Analysis was performed within 5 minutes over a good linear concentration range from
2.4 ng/mL to 48 ng/mL (r2 = 0.9993 ± 0.018) for verapamil and 10pg/mL to 200pg/mL (r2
=0.9993± 0.006) for trandolapril .The extraction recoveries and matrix effect of verapamil and
trandolapril were 98.45, 99.95, 98.12, 99.66% and 98.27, 99.89, 97.78, 99.23% respectively, at different
QC concentration levels. Precision and recovery study results were determined within the
acceptable limit. An electrospray ionization source was used to study verapamil and Trandolapril at
m/z 454.72→182.16, 430.25→201.48, and IS for m/z 460.18→ 324.39, 436.28 → 340.52, which
were ion pairs of mass analysis. This method has successfully been applied to explore verapamil
(1.2mg/kg) with its internal standard (D6-Verapamil), trandolapril (0.005 mg/kg) with its internal
standard (D6-Trandolapril) extracted from rat plasma using liquid-liquid extraction.
Conclusion:
This manuscript focuses on the consistent evaluation of the key bioanalytical validation
parameters, and the following are discussed: accuracy, precision, sensitivity, selectivity,
standard curve, limits of quantification, range, recovery, and stability. These validation parameters
are described, together with illustrations of validation methodology applied in the case of
chromatographic methods used in bioanalysis.
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmaceutical Science,Molecular Medicine,Biochemistry,Biophysics
Cited by
2 articles.
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