Minocycline Increases in-vitro Cortical Neuronal Cell Survival after Laser Induced Axotomy

Author:

Yulug Burak1,Ozansoy Mehmet2,Alokten Merve2,Ozansoy Muzaffer B.C.2,Cankaya Seyda1,Hanoglu Lutfu3,Kilic Ulkan4,Kilic Ertugrul5

Affiliation:

1. Department of Neurology, Faculty of Medicine, Alanya Alaaddin Keykubat University, Antalya/Alanya, Turkey

2. Istanbul Medipol University, Regenerative and Restorative Medicine Research Center, Istanbul, Turkey

3. Department of Neurology, Istanbul, Faculty of Medicine, Medipol University, Istanbul, Turkey

4. Department of Medical Biology, University of Health Sciences, Faculty of Medicine, Istanbul, Turkey

5. Department of Physiology, Istanbul Medipol University, School of Medicine, Istanbul, Turkey

Abstract

Background: Antibiotic therapies targeting multiple regenerative mechanisms have the potential for neuroprotective effects, but the diversity of experimental strategies and analyses of non-standardised therapeutic trials are challenging. In this respect, there are no cases of successful clinical application of such candidate molecules when it comes to human patients. Methods: After 24 hours of culturing, three different minocycline (Sigma-Aldrich, M9511, Germany) concentrations (1 μM, 10 μM and 100 μM) were added to the primary cortical neurons 15 minutes before laser axotomy procedure in order to observe protective effect of minocycline in these dosages. Results: Here, we have shown that minocycline exerted a significant neuroprotective effect at 1 and 100μM doses. Beyond confirming the neuroprotective effect of minocycline in a more standardised and advanced in-vitro trauma model, our findings could have important implications for future studies that concentrate on the translational block between animal and human studies. Conclusion: Such sophisticated approaches might also help to conquer the influence of humanmade variabilities in critical experimental injury models. To the best of our knowledge, this is the first study showing that minocycline increases in-vitro neuronal cell survival after laser-axotomy.

Publisher

Bentham Science Publishers Ltd.

Subject

Pharmacology (medical),General Pharmacology, Toxicology and Pharmaceutics,General Medicine

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