Affiliation:
1. Institute of Molecular Science, Shanxi University, Taiyuan 030006, China
Abstract
Objective:
The work aimed to compare the binding between the two main components of
Polygala tenuifolia Willd. and two cholinesterases (ChEs) by using a variety of spectral techniques.
Methods:
Two main components of Polygala tenuifolia Willd. included Tenuifolin (Ten) and
Onjisaponin B (Onj B), and two ChEs included acetylcholinesterase (AChE) and butyrylcholinesterase
(BChE).
Results:
The UV-visible absorption spectra results showed that Ten had no effect on the structure of
ChEs, and the combination of Onj B with ChEs changed its structure. Onj B statically quenched the
endogenous fluorescence of both of ChEs, Ten dynamically quenched the endogenous fluorescence of
AChE with no effect on BChE. The fluorescence quenching rate of ChEs by Onj B was much higher
than that of AChE by Ten, and only one binding site of each protein spontaneously interacted with the
compound to bind to or collide. Synchronous fluorescence results showed that Ten and Onj B
quenched the fluorescence intensity by affecting tryptophan and tyrosine residues in cholinesterases,
respectively. Hydrophobic force played an important role in the interaction between Ten and AChE,
and van der Waals force and hydrogen bond were the main driving forces for the binding of Onj B to
ChEs. The Enzyme activity test showed that Onj B inhibited ChE activity, and Ten never inhibited
ChE activity.
Conclusion:
Onj B has the potential to inhibit ChE activity and increase the neurotransmitter
acetylcholine content in the nerve system, improving the Alzheimer's disease (AD).
Funder
Students Research Training supported the work for undergraduates of Shanxi University
Publisher
Bentham Science Publishers Ltd.
Subject
Biochemistry,General Medicine,Structural Biology