Affiliation:
1. Department of General Surgery, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and
Science, Xiangyang, 441021, Hubei, China
2. Department of Coloproctological Surgery, Xiangyang Central Hospital,
Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, 441021, Hubei, China
Abstract
Background:
Neurensin-2 (NRSN2) is reported to be associated with the progression of
many tumors. This work aimed at investigating the biological function and prognostic significance of
NRSN2 in gastric cancer (GC).
Methods:
NRSN2 expression in various cancer tissue was analyzed by the TIMER database. NRSN2
expression in GC tissue samples of different groups was analyzed by the UALCAN database. The
survival analysis was performed with the Kaplan-Meier database. NRSN2 expression in GC tissues
and cell lines was measured by qRT-PCR and Western blot. CCK-8, Transwell and scratch healing
assays were conducted to detect the proliferative, migrative and invasive capabilities of GC cells,
respectively. The LinkedOmics database and StarBase database were utilized to analyze the related
genes with NRSN2 in GC. The association of NRSN2 expression with tumor immune infiltrating cells
and molecular markers of immune cells was investigated with the TIMER database.
Results:
NRSN2 expression was up-regulated in GC tissues, which was correlated with GC tumor
grade, lymph node metastasis, and TP53 mutation. The prognosis of GC patients with high NRSN2
expression was worse than those of the patients with low NRSN2 expression. NRSN2 expression was
also associated with the TNM stage, and Lauren subtype of GC patients. NRSN2 overexpression
promoted the growth, migration and invasion of GC cells lines; knocking down NRSN2 worked
oppositely. NRSN2 expression in GC was associated with Wnt, p53, and NOD-like receptor signaling
pathways. NRSN2 expression was also significantly associated with the infiltration of CD8+ T cells,
CD4+ T cells, macrophages, neutrophils, and dendritic cells in the GC microenvironment.
Conclusion:
NRSN2 expression in GC tissues is up-regulated, which correlates with a poor prognosis
and immune cell infiltration of GC patients. NRSN2 facilitates the growth and aggressiveness of GC
cells, implying that it may be a diagnostic biomarker and therapy target for GC.
Publisher
Bentham Science Publishers Ltd.
Subject
Biochemistry,General Medicine,Structural Biology
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