Affiliation:
1. Department of Cardiovascular medicine, The Third Affiliated Hospital of Zunyi Medical University (The First People’s
Hospital of Zunyi), Zunyi, Guizhou, 563002, P.R. China
Abstract
Background:
Studies have found that microRNAs (miRNAs) participate in the pathogenesis
of myocardial ischemia-reperfusion injury (MIRI). miR-330-5p alleviated cerebral IR injury
and regulated myocardial damage. However, the mechanism of the effect of miR-330-5p on MIRI
needs to be further studied.
Objective:
The study aimed to explore the role and mechanism of miR-330-5p in MIRI.
Methods:
The oxygen-glucose deprivation reperfusion (OGD/R) model was constructed in cardiomyocytes
to simulate MIRI in vitro. QRT-PCR was used for the detection of gene expression.
ELISA was used for evaluation of the levels of aldehyde dehydrogenase 2 family member (ALDH2),
4-hydroxynonenal (4-HNE), and malondialdehyde (MDA). Flow cytometry was used to evaluate
apoptosis. Western blot was employed for protein determination. Bioinformatic analysis was
performed for predicting the targets of miR-330-5p.
Results:
miR-330-5p was found to be down-regulated in MIRI-induced cardiomyocytes (Model
group). miR-330-5p mimic enhanced ALDH2 activity, inhibited apoptosis, and suppressed 4-HNE
and MDA of MIRI-induced cardiomyocytes. miR-330-5p inhibited ERK expression while increasing
the p38 expression. Bioinformatic analysis showed hydroxysteroid 11-beta dehydrogenase 1
(HSD11B1) to be a target of miR-330-5p. HSD11B1 expression was inhibited by miR-330-5p mimic
while increased by miR-330-5p inhibitor in MIRI-induced cardiomyocytes. HSD11B1 overexpression
reversed the effect of miR-330-5p on ALDH2, 4-HNE, MDA, apoptosis, and ERK/p38
signaling pathway. Furthermore, lncRNA small nucleolar RNA host gene 3 (SNHG3) was the upstream
lncRNA of miR-330-5p. SNHG3 decreased miR-330-5p expression and increased HSD11B1
expression.
Conclusion:
SNHG3/miR-330-5p alleviated MIRI in vitro by targeting HSD11B1 to regulate the
ERK/p38 signaling pathway.
Funder
Zunyi Science and Technology Bureau Joint Fund
Publisher
Bentham Science Publishers Ltd.
Subject
Biochemistry,General Medicine,Structural Biology
Cited by
1 articles.
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