Author:
Radchenko Olexiy M.,Sandetska Nadia V.,Morgun Bogdan V.,Karelov Anatolii,Kozub Natalia,Pirko Yaroslav V.,Blume Yaroslav B.
Abstract
Background:
The allelic composition of the gene Lr34/Yr18/Sr57/Pm38/Bdv1, which is associated with resistance to leaf rust in varieties of bread wheat (Triticum aestivum L.), has been investigated.
Methods:
Three DNA markers were used to determine the allelic state of the gene Lr34/Yr18/Sr57/Pm38/Bdv1: the co-dominant molecular genetic markers cssfr5 and csLV34 and the microsatellite marker Xgwm295.
Results:
Among 32 cultivars evaluated for resistance to leaf rust, 4 were highly resistant, 26 were resistant and 2 were moderately susceptible. Using the co-dominant marker cssfr5 based on the detection of the polymorphic state of one of the exons of the gene Lr34/Yr18/Sr57/Pm38/Bdv1, the Lr34(+) allele, which confers resistance to leaf rust, was found in 25% of the studied varieties. The coincidence between the results obtained with the markers cssfr5 and csLV34 was 84.5%.
Conclusion:
The data of the conducted molecular genetic analysis were supplemented by observations of the resistance of the studied varieties to leaf rust in the field. The obtained data can be used in breeding programs to develop new varieties and breeding lines with leaf rust resistance.
Publisher
Bentham Science Publishers Ltd.
Subject
Soil Science,Agronomy and Crop Science,Animal Science and Zoology
Reference34 articles.
1. Karelov AV, Pirko YV, Kozub NA, et al.
Identification of the allelic state of the Lr34 leaf rust resistance gene in soft winter wheat cultivars developed in Ukraine.
Cytol Genet
2011;
45
(5)
: 271-6.
2. Morgounov A, Tufan HA, Sharma R, et al.
Global incidence of wheat rusts and powdery mildew during 1969–2010 and durability of resistance of winter wheat variety Bezostaya 1.
Eur J Plant Pathol
2012;
132
(3)
: 323-40.
3. McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC.
Сatalogue of gene symbols for wheat: 2017 supplement.
2017.
https://shigen.nig.ac.jp/wheat/komugi/ genes/macgene/supplement2017.pdf
4. Kokhmetova A, Rsaliyev S, Atishova M, et al.
Evaluation of wheat germplasm for resistance to leaf rust (Puccinia triticina) and identification of the sources of Lr resistance genes using molecular markers.
Plants
2021;
10
(7)
: 1484.
5. Babu P, Baranwal DK, Harikrishna , et al.
Application of genomics tools in wheat breeding to attain durable rust resistance.
Front Plant Sci
2020;
11
: 567147.
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