Synthesis and Evaluation of Isatin Schiff Bases Against Plant Pathogens Validated Through Aspartyl Protease and Acetylcholine Binding Proteins Docked Studies

Abstract

Schiff bases of isatin were synthesized by reacting isatin with substituted aromatic amines and were characterized by UV-Visible, 1HNMR, 13CNMR, IR and micro analytical data. All the synthesized isatin Schiff bases were screened in vitro against wheat pathogenic fungi Bipolaris sorokiniana, Alternaria triticina using spore inhibition technique and brinjal parasite- Meloidogyne incognita by egg hatch inhibition and J2 mortality. The in vitro study and docking simulation studies revealed that the 3-(2, 4, 5-trichlorophenylimino) indolin-2-one 6f and 3-(2, 4- dinitrophenylimino) indolin-2-one 6c substituted with tri-halogen and dinitro electron withdrawing groups were found to be promising antipathogenic candidates. The possible binding interactions of tested compounds with Aspartyl protease and Acetylcholine binding proteins were analyzed through molecular docking.