Affiliation:
1. Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang 453003, Henan, China
Abstract
Background:
Colon cancer is one of the most common malignant tumors, and B cell Translocation
Gene (BTG)1 is involved in the occurrence and development of colon cancer, however, the
underlying molecular mechanism remains unclear.
Objective:
In this study, we investigated the expression of BTG1 protein in colon cancer, and its association
with clinicopathology and prognosis.
Methods:
The tumor specimens from 59 patients with colon cancer who had undergone radical colectomy
were selected as the observation group. Para-carcinoma tissues from the same patients were selected
as the control group. The expressions of BTG1 mRNA and protein in the specimen of two
groups were analyzed by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Western
blot. According to the immunohistochemical results, the patients were divided into BTG1-negative and
BTG1-positive groups. The postoperative cumulative survival rate in the two groups was analyzed. The
association of the expression of BTG1 protein with the clinicopathological features and postoperative
survival was investigated.
Results:
Compared with the control group, the expression levels of BTG1 mRNA and BTG1 protein
were significantly decreased in the observation group (P < 0.05). Immunohistochemical analysis revealed
that there were 12 positive tumor samples and 47 negative samples. The expression of BTG1
was negatively associated with the degree of differentiation and lymphatic metastasis. The cumulative
survival rate of BTG1-positive patients was significantly increased compared with that of BTG1-
negative patients (P < 0.05). Stepwise Cox regression analysis showed that lymphatic metastasis, tumor
size and BTG1 expression level were independent prognostic factors for overall survival in patients
with colon cancer.
Conclusion:
BTG1 protein in colon cancer tissues were expressed at low levels, which was associated
with the clinicopathological features, postoperative recurrence and survival of patients.
Funder
Education Department of Henan Province
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmacology (medical),Cancer Research,Drug Discovery,Oncology,General Medicine
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