Author:
Arango Carolina,Acosta-Gonzalez Alejandro,Parra-Giraldo Claudia M.,Sánchez-Quitian Zilpa A.,Kerr Russell,Díaz Luis E.
Abstract
Introduction:New strategies have been arisen to set a rapid and effective screening for selection of microorganism with bioactive potential. This study suggests that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.Material and Methods:An unstudied microbial community from a tropical river sediment in Colombian Orinoquía is described, applying an extended cultivation strategy using physicochemical pretreatments, biological screenings and taxonomic dereplication through MALDI-TOF MS approach.Results:Actinobacteria-like isolates (790) were growth and their antimicrobial activity was assessed against methicillin-resistantStaphylococcus aureus, Vancomycin-resistantEnterococcus faecium, extended-spectrum β-lactamaseKlebsiella pnumoniae, and clinical isolates ofCladosporium cladosporioidesandEpicoccum nigrum.Seventy-eight isolates, belonging to the Streptomycetaceae family according to 16S rDNA analysis were found to have antimicrobial activity and were categorized as low abundance actinobacteria by MALDI-TOF MS.Conclusion:The results suggest that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents.
Publisher
Bentham Science Publishers Ltd.
Subject
General Immunology and Microbiology
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