Listeriolysin O, but not Murine E-cadherin, is Involved in Invasion of Listeria monocytogenes into Murine Liver Parenchymal Cells

Abstract

Human E-cadherin and listeriolysin O (LLO) are involved in invasion ofListeria monocytogenesinto human liver parenchymal cells (LPC). Yet, it remains to be determined whether murine E-cadherin and LLO participate in invasion ofL. monocytogenesinto murine LPC. In the present study, involvement of murine E-cadherin and LLO in invasion ofL. monocytogenesinto murine LPC was investigated. Murine E-cadherin was expressed on murine LPC, but the expression became undetectable by insertion of transgene of Simian virus 40 large T antigen. Although invasion ofL. monocytogenesinto murine LPC was found regardless of murine E-cadherin expression, infection rate ofL. monocytogenesbeing unable to secrete LLO was lower than that ofL. monocytogenesbeing capable of secreting LLO. Our RESULTS verify that invasion ofL. monocytogenesinto murine LPC occurs independently of murine E-cadherin and indicate that LLO participates in invasion ofL. monocytogenesinto murine LPC.