Author:
Scarduelli Marcelo,Guizelini Dieval,Alves Cardos Rodrigo Luis,Ceccon Denny Marcel,Donatti Lucélia,de Baura Valter Antônio,de Oliveira Pedrosa Fábio,Huergo Luciano Fernandes,de Souza Emanuel Maltempi
Abstract
Background:
Bacillus safensis BRM1 was isolated from Brazilian mangrove sediment and selected for its ability to grow in xylan as the sole carbon source. To identify genes encoding biomass conversion enzymes, the genome of this bacterium was sequenced.
Methods:
Genome wide analysis revealed 99% nucleotide identity to the Bacillus safensis genome. The isolated strain was named B. safensis BRM1, and its genome consists of a circular chromosome of 3.74 Mb with a GC content of 41.8%. Genes encoding a plethora of hydrolytic enzymes are present in the BRM1 genome but absent from the other B. safensis genomes.
Results:
A total of 23 genes encoding putative cellulases or hemicellulases were identified.
Conclusion:
These data support that B. safensis BRM1 is an interesting candidate for the prospection of enzymes that can be applied in the conversion of cellulosic biomass to biofuel.
Publisher
Bentham Science Publishers Ltd.
Subject
General Immunology and Microbiology