Affiliation:
1. Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, India
2. Dr. D. Y. Patil Vidyapeeth, Pimpri, Pune, India
3. Regenerative Medicine Laboratory, Dr. D. Y. Patil Dental College
and Hospital, Pimpri, Pune, India
Abstract
Background:
Diabetes occurs due to insulin deficiency or less insulin. To manage this condition,
insulin administration as well as increased insulin sensitivity is required, but exogeneous insulin
cannot replace the sensitive and gentle regulation of blood glucose levels same as β cells of healthy
individuals. By considering the ability of regeneration and differentiation of stem cells, the current
study planned to evaluate the effect of metformin preconditioned buccal fat pad (BFP) derived mesenchymal
stem cells (MSCs) on streptozotocin (STZ) induced diabetes mellitus in Wistar rats.
Materials & Methods:
The disease condition was established by using a diabetes-inducing agent STZ
in Wistar rats. Then, the animals were grouped into disease control, blank, and test groups. Only the
test group received the metformin-preconditioned cells. The total study period for this experiment was
33 days. During this period, the animals were monitored for blood glucose level, body weight, and
food-water intake twice a week. At the end of 33 days, the biochemical estimations for serum insulin
level and pancreatic insulin level were performed. Also, histopathology of the pancreas, liver and
skeletal muscle was performed.
Results:
The test groups showed a decline in the blood glucose level and an increase in the serum pancreatic
insulin level as compared to the disease group. No significant change in food and water intake
was observed within the three groups, while body weight was significantly reduced in the test group
when compared with the blank group, but the life span was increased when compared with the disease
group.
Conclusion:
In the present study, we concluded that metformin preconditioned buccal fat pad-derived
mesenchymal stem cells have the ability to regenerate damaged pancreatic β cells and have antidiabetic
activity, and this therapy is a better choice for future research.
Publisher
Bentham Science Publishers Ltd.