Affiliation:
1. Department of Microbiology, Islamic Azad University, Qom Branch, Qom, Iran
2. Razi Herbal Medicines Research
Center, Lorestan University of Medical Sciences, Khorramabad, Iran
3. Department of Biology, Faculty of Basic
Science, Islamic Azad University, Central Tehran Branch, Tehran, Iran
Abstract
Background:
Nanoparticle biology is preferable to other common methods due to its
economic efficiency and compatibility with the environment. On the other hand, the prevalence of
drug-resistant bacterial strains is expanding and it is necessary to use alternative antibiotic compounds
to deal with them. The aim of the present study was the biosynthesis of zinc oxide nanoparticles(ZnO
NPs) by Lactobacillus spp. and their antimicrobial effect.
Methods:
In this study, after the biosynthesis of ZnO NPs by Lactobacillus spp, Characterization of
Nanoparticulation Was performed by UV–Vis, XRD, and Scanning Electron Microscopy (SEM). Additionally,
Lactobacillus spp. - ZnO NPs were assessed for their antimicrobial properties.
Results:
UV-visible spectroscopy confirmed the Lactobacillus spp. - ZnO NPs absorbed UV in the
region of 300-400 nm. XRD analysis showed the presence of zinc metal in nanoparticles. SEM
revealed that Lactobacillus plantarum - ZnO NPs were smaller than the others. Staphylococcus aureus
showed the largest non-growth halo diameter against ZnO NPs synthesized by L. plantarum ATCC
8014 (3.7 mm). E. coli had the largest growth halo diameter against ZnO NPs synthesized by L. casei
(3 mm) and L. plantarum (2.9 mm). The MIC values of ZnO NPs synthesized by L. plantarum ATCC
8014, L.casei ATCC 39392, L. fermenyum ATCC 9338, L. acidophilus ATCC 4356 were 2,8,8 and 4
μg/mL for Staphylococcus aureus. The MIC values of ZnO NPs synthesized by L. plantarum ATCC
8014, L. casei ATCC 39392, L. fermenyum ATCC 9338, L. acidophilus ATCC 4356 were 2, 4, 4, and
4 μg/ml for E. coli. The lowest MICs were 2 μg/ml for E. coli and S. aureus related to ZnO NPs
synthesized by L. plantarum ATCC 8014. MIC and MBC values were equivalent to each other.
Conclusion:
The results of this research show that ZnO NPs synthesized by L. plantarum ATCC 8014
have more antimicrobial effects than other ZnO NPs used. Therefore, the ZnO NPs made with Lactobacillus
plantarum ATCC 8014 have the potential to kill bacteria and can be considered a candidate
for antibiotic replacement.
Publisher
Bentham Science Publishers Ltd.