Affiliation:
1. The First Affiliated Hospital of Zhejiang Chinese Medical
University, Hangzhou, Zhejiang, 310006, P.R. China
2. College of Pharmaceutical Science, Zhejiang Chinese Medical
University, Hangzhou, Zhejiang 310053, P.R. China
3. Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of
Technology, Hangzhou, Zhejiang, 310014, P.R. China
Abstract
Aim:
The aim of the study was to explore the efficacy as well as the mechanism of action
of Pitongshu (PTS) on rats with functional dyspepsia (FD) induced by iodoacetamide gavage and
tail clamping.
Methods:
The bioactive components of PTS were obtained from the Traditional Chinese Medicine
Systems Pharmacology Database and Analysis Platform (TCMSP), whereas the potential targets of
PTS were obtained from the Similarity Ensemble Approach (SEA), TCMSP, and Swiss Target Prediction
Database. The disease targets were obtained from the DisGeNET database, whereas Gene
Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were
performed using the R Software. The method of iodoacetamide gavage combined with tail clamping
was used to establish the FD rat model in this study. Body weight, food intake, gastrointestinal motility,
gastric acidity and secretion, and the mechanical pain threshold of rats were measured. The
open-field test was also performed. The stomach and duodenum were histologically observed. The
levels of serotonin (5-HT), Calcitonin Gene-Related Peptide (CGRP), Motilin (MTL), and Gastrin
(GAS) in gastric tissues were detected by ELISA.
Results:
A total of 139 bioactive components and 17 potential targets of PTS were identified through a
network pharmacology approach. The results of GO and KEGG enrichment analyses indicated that
PTS could reduce the 5-HT secretion of gastric tissues through the serotonergic synaptic pathway and
alleviate the symptoms of FD, indicating that PTS plays a therapeutic role. The results of animal experiments
showed that PTS could increase body weight and food intake, improve autonomous activity,
and decrease gastric acidity and secretion in FD rats. Furthermore, gastric sensitivity increased in FD
rats, and PTS treatment could significantly decrease it. The results of ELISA showed that the overexpression
of 5-HT and CGRP was decreased after PTS treatment in FD rats. Lastly, PTS could significantly
improve gastrointestinal motility, as well as the levels of GAS and MTL in FD rats.
Conclusion:
PTS may reduce 5-HT secretion by regulating the serotonergic synaptic pathway,
thereby reducing visceral sensitivity and alleviating the symptoms of FD.
Publisher
Bentham Science Publishers Ltd.
Subject
Organic Chemistry,Computer Science Applications,Drug Discovery,General Medicine
Cited by
4 articles.
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