Affiliation:
1. Nanjing Drum Tower Hospital, Clinical College of Xuzhou Medical University, Xuzhou, China
2. Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong, China
3. Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China
Abstract
Background:
Acute Liver Failure (ALF) is a difficult problem to solve in clinical practice.
The presence of non-SMC condensin I complex subunit G (NCAPG) has previously been
linked to vascular invasion of digestive system tumors, foreshadowing poor prognosis. Its role in
ALF biology, however, remains unknown. This article explores the role of NCAPG as a potential
biomarker candidate for the accurate diagnosis and targeted treatment of ALF.
Methods:
The study included transcription data (GSE14668, GSE38941, GSE62029, GSE96851,
and GSE120652) of ALF, normal tissues, and clinical samples, where NCAPG was selected as the
differential gene by the “DESeq2” R package to analyze the immune cell functions and signal
pathways. Furthermore, RT-qPCR and Western blot analyses were used to confirm the RNA and
protein levels of NCAPG in ALF cell models, respectively.
Results:
Bioinformatics analysis revealed that NACPG was up-regulated in ALF tissues, and the
functional signaling pathway was primarily associated with immune infiltration. Based on the results
of clinical samples, we suggest that NCAPG was overexpressed in ALF tissues. We also
found that the expression of NCAPG increased with the degree of liver injury in vitro. Enrichment
analysis suggested that NCAPG influenced ALF as a PI3K/AKT pathway activator.
Conclusion:
Our study suggests that NCAPG is a preliminary tool for the diagnosis of ALF. It can
affect ALF via the PI3K/AKT pathway and is a potential therapeutic target to improve prognosis.
Funder
Fundamental Research Funds for the Central Universities
Nanjing health science and technology development project for Distinguished Young Scholars
Aid project of Nanjing Drum Tower Hospital Health, Education & Research Foundation
Affiliated Drum Tower Hospital, Medical School of Nanjing University
Chen Xiao-ping Foundation for the Development of Science and Technology of Hubei Province, China
Publisher
Bentham Science Publishers Ltd.
Subject
Organic Chemistry,Computer Science Applications,Drug Discovery,General Medicine